Abstract Changes in the physiological state and adhesion of probiotics under stresses affect their interaction with the host. The effects of osmotic (3% NaCl, 6% NaCl, and 3% NaCl + 3% KCl), acid (pH 5.0), and alkali (pH 8.0) stress on the physiological characteristics, adhesion ability and pathogen adhesion inhibition of Lactobacillus plantarum KLDS 1.0328 were investigated. The results showed that 6% NaCl resulted in lower acid production, growth and antimicrobial activity of cells compared to control and 3% NaCl treatment. Reduced surface hydrophobicity, aggregation ability, adhesion ability and pathogen adhesion inhibition were observed when L. plantarum KLDS 1.0328 was exposed to 6% NaCl. These changes were weakened when NaCl was partially replaced by KCl. Exposure to stresses other than alkali stress significantly increased the ratio of unsaturated fatty acid to saturated fatty acid in the cell membrane. The autoaggregation and adhesion ability of cells were increased under pH 5.0 treatment. The results demonstrated that the abilities of cells to adhere and inhibit pathogen adhesion were significantly positively correlated with the ability to coaggregate with pathogens. This study provides a basis for our understanding of the response of L. plantarum to stresses and the related molecular mechanisms.

中文翻译：

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环境胁迫对植物乳杆菌KLDS 1.0328生理特性、粘附能力及病原菌粘附抑制的影响

摘要 应激下益生菌生理状态和粘附的变化会影响它们与宿主的相互作用。渗透性（3% NaCl、6% NaCl、3% NaCl + 3% KCl）、酸（pH 5.0）、碱（pH 8.0）胁迫对植物乳杆菌生理特性、粘附能力和病原菌粘附抑制的影响研究了KLDS 1.0328。结果表明，与对照和 3% NaCl 处理相比，6% NaCl 导致细胞的产酸、生长和抗菌活性较低。当植物乳杆菌 KLDS 1.0328 暴露于 6% NaCl 时，观察到降低的表面疏水性、聚集能力、粘附能力和病原体粘附抑制。当 NaCl 被 KCl 部分取代时，这些变化会减弱。暴露于碱胁迫以外的胁迫显着增加了细胞膜中不饱和脂肪酸与饱和脂肪酸的比率。在 pH 5.0 处理下，细胞的自聚集和粘附能力增加。结果表明，细胞粘附和抑制病原体粘附的能力与与病原体共聚的能力呈显着正相关。该研究为我们了解植物乳杆菌对胁迫的反应及其相关分子机制提供了基础。结果表明，细胞粘附和抑制病原体粘附的能力与与病原体共聚的能力呈显着正相关。该研究为我们了解植物乳杆菌对胁迫的反应及其相关分子机制提供了基础。结果表明，细胞粘附和抑制病原体粘附的能力与与病原体共聚的能力呈显着正相关。该研究为我们了解植物乳杆菌对胁迫的反应及其相关分子机制提供了基础。