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Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis
Electroanalysis ( IF 2.7 ) Pub Date : 2020-01-29 , DOI: 10.1002/elan.201900697 Shimaa Eissa 1 , Rahmah Noordin 2 , Mohammed Zourob 1, 3
Electroanalysis ( IF 2.7 ) Pub Date : 2020-01-29 , DOI: 10.1002/elan.201900697 Shimaa Eissa 1 , Rahmah Noordin 2 , Mohammed Zourob 1, 3
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Cystic echinococcosis (CE) or hydatid disease is a parasitic infection caused by Echinococcus granulosus . Early serodiagnosis and continuous monitoring of the disease is very important for medical treatment. Here, we report the detecting of both echinococcus antigen and antibody for the diagnosis of hydatid disease using square wave voltammetry (SWV)‐based immunosensors. The gold electrodes were functionalized using cysteamine/phenylene diisothiocyanate linkers and used for the immunosensors fabrication. The hydatid antigen and antibody immunosensors were constructed by the immobilization of either purified rabbit polyclonal antibody or recombinant antigen B (AgB), respectively on the functionalized gold electrodes surfaces. The detection in both cases was achieved by following the change in the SWV reduction peak current of the ferro/ferricyanide redox couple upon antibody or antigen binding. These immunosensors enabled the detection of echinococcus antigen and antibody within a concentration range of 1 pg.mL−1 to 1 μg.mL−1 with detection limits of 0.4 pg.mL−1 and 0.3 pg.mL−1, respectively. A preliminary application of the developed immunosensor was performed in spiked serum sample showing good recovery percentages ranging from 102 to 110 % for both hydatid antibody and antigen detection. This easy‐to‐use, sensitive, and low cost quantitative method holds great promise for the early diagnosis of hydatid disease and thus, better managements and treatment outcomes.
中文翻译：
无伏安标记的免疫传感器用于囊性棘球E病的诊断
囊性棘球co虫病（CE）或hy虫病是由细粒棘球caused虫病引起的寄生虫感染。疾病的早期血清诊断和持续监测对于药物治疗非常重要。在这里，我们报告使用基于方波伏安法（SWV）的免疫传感器检测棘球disease病的棘球oc虫抗原和抗体。金电极使用半胱胺/亚苯基二异硫氰酸酯接头进行功能化，并用于免疫传感器的制造。d虫抗原和抗体免疫传感器分别通过将纯化的兔多克隆抗体或重组抗原B（AgB）固定在功能化的金电极表面上而构建。两种情况下的检测均通过跟踪抗体或抗原结合后铁/铁氰化物氧化还原对的SWV降低峰值电流的变化来实现。-1至1μg.mL - 1，检测极限分别为0.4 pg.mL -1和0.3 pg.mL -1。所开发的免疫传感器的初步应用是在加标血清样品中进行的，显示出良好的回收率，适用于包虫抗体和抗原检测，回收率在102％至110％之间。这种易于使用，灵敏且低成本的定量方法为包虫病的早期诊断以及更好的管理和治疗结果提供了广阔的前景。
更新日期：2020-01-29
中文翻译：
无伏安标记的免疫传感器用于囊性棘球E病的诊断
囊性棘球co虫病（CE）或hy虫病是由细粒棘球caused虫病引起的寄生虫感染。疾病的早期血清诊断和持续监测对于药物治疗非常重要。在这里，我们报告使用基于方波伏安法（SWV）的免疫传感器检测棘球disease病的棘球oc虫抗原和抗体。金电极使用半胱胺/亚苯基二异硫氰酸酯接头进行功能化，并用于免疫传感器的制造。d虫抗原和抗体免疫传感器分别通过将纯化的兔多克隆抗体或重组抗原B（AgB）固定在功能化的金电极表面上而构建。两种情况下的检测均通过跟踪抗体或抗原结合后铁/铁氰化物氧化还原对的SWV降低峰值电流的变化来实现。-1至1μg.mL - 1，检测极限分别为0.4 pg.mL -1和0.3 pg.mL -1。所开发的免疫传感器的初步应用是在加标血清样品中进行的，显示出良好的回收率，适用于包虫抗体和抗原检测，回收率在102％至110％之间。这种易于使用，灵敏且低成本的定量方法为包虫病的早期诊断以及更好的管理和治疗结果提供了广阔的前景。
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