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Type 3 adenylyl cyclase in the MOE is involved in learning and memory in mice.
Behavioural Brain Research ( IF 2.6 ) Pub Date : 2020-02-02 , DOI: 10.1016/j.bbr.2020.112533
Xinxia Liu 1 , Yanfen Zhou 2 , Dong Yang 2 , Shujuan Li 2 , Xiaodong Liu 3 , Zhenshan Wang 2
Affiliation  

Although olfactory dysfunction is related to learning and memory impairment, the causal relationship between main olfactory epithelium (MOE) disruption and learning and memory is still unknown. The present study aimed to establish whether MOE disruption causes learning and memory impairment and whether the expression of type 3 adenylyl cyclas (AC3) in the MOE is related to learning and memory. First, the buried food test was carried out to confirm that MOE function was disrupted in mice treated with nasal instillation of zinc sulfate (ZnSO4 mice), and mice with specific knockdown of AC3 in the MOE by CRISPR/Cas9 technology (AC3KD/MOE mice). Then, behavioural tasks associated with learning and memory were administered. ZnSO4 mice and AC3KD/MOE mice showed impairments in learning and memory tests, including the novel object recognition test, the step-down passive avoidance test, the Morris water maze test, and the Y-maze test. Our data demonstrate that MOE disruption caused by nasal exposure to ZnSO4 or specific knockdown of AC3 in the MOE resulted in learning and memory impairment, and they further demonstrate that the expression of AC3 in the MOE plays a major role in learning and memory.

中文翻译:

MOE中的3型腺苷酸环化酶参与小鼠的学习和记忆。

尽管嗅觉功能障碍与学习和记忆障碍有关,但主要嗅觉上皮(MOE)破坏与学习和记忆之间的因果关系仍然未知。本研究旨在确定MOE破坏是否会引起学习和记忆障碍,以及MOE中3型腺苷酸环(AC3)的表达是否与学习和记忆有关。首先,进行了掩埋食物测试,以确认经鼻滴注硫酸锌治疗的小鼠(ZnSO4小鼠)和通过CRISPR / Cas9技术在MOE中具有特定敲低AC3作用的小鼠(AC3KD / MOE小鼠)的MOE功能受到破坏)。然后,管理与学习和记忆有关的行为任务。ZnSO4小鼠和AC3KD / MOE小鼠在学习和记忆测试(包括新型物体识别测试)中显示出障碍,降压被动回避测试,莫里斯水迷宫测试和Y迷宫测试。我们的数据表明,经鼻暴露于ZnSO4引起的MOE破坏或MOE中AC3的特异性敲低导致学习和记忆障碍,并且他们进一步证明,MOE中AC3的表达在学习和记忆中起主要作用。
更新日期:2020-02-03
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