Abstract Iron is released following electrochemical reduction of immobilized ferritin. In this work, voltammetry is used to elucidate the identity of the released iron. Ferritin was covalently immobilized on 6-mercaptohexanoic acid modified gold electrodes. The current-potential curve shows an initial cathodic peak indicating the reduction of ferritin. The return scan gives an anodic peak that is probably due to the oxidation of released Fe(H2O)62+. The second cycle shows a new cathodic wave suggesting the reduction of the iron species. Voltammetry, while stirring the cell solution, reveals that the released iron is dissolved while the oxidized form is immobilized. The cathodic peak potential of the oxidized iron species has a dependence of 120 mV/pH indicating a two-proton exchange per electron transferred, suggesting that Fe(H2O)62+ is oxidized to Fe(OH)2+. Results from voltammetric scans in the presence of hesperitin, Trolox, and maltol further suggest that the oxidized form of iron is Fe(OH)2+.

中文翻译：

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固定在自组装单层修饰金电极上的铁蛋白释放的铁物种的表征

摘要 铁在固定化铁蛋白的电化学还原后释放。在这项工作中，伏安法用于阐明释放的铁的身份。铁蛋白共价固定在 6-巯基己酸修饰的金电极上。电流-电位曲线显示初始阴极峰，表明铁蛋白减少。返回扫描给出了一个阳极峰，这可能是由于释放的 Fe(H2O)62+ 的氧化所致。第二个循环显示了一个新的阴极波，表明铁种类减少了。伏安法在搅拌细胞溶液时显示释放的铁溶解而氧化形式被固定。氧化铁物质的阴极峰电位具有 120 mV/pH 的依赖性，表明每个电子转移有两个质子交换，表明 Fe(H2O)62+ 被氧化为 Fe(OH)2+。在橙皮素、Trolox 和麦芽酚存在下的伏安扫描结果进一步表明铁的氧化形式是 Fe(OH)2+。