Abstract The aroma fraction of fresh fruits is a key factor to evaluate quality and acceptance or resistance of customers. Due to the limited sensitivity of current analytical methods, many important aroma components are unidentified in most studies of fresh fruit. In this work, the aroma compounds in the fresh navel orange were studied by multidimensional gas chromatography quadrupole time-of-flight mass spectrometry which can simultaneously achieve one-dimensional and comprehensive two-dimensional separation on only one instrument, with no need to change columns. As a result, a total of 97 volatile compounds were determined by comprehensive two-dimensional gas chromatography while only 44 compounds were identified by one-dimensional gas chromatography. The identified compounds, which account for 98.30% of the volatile chemicals present in the orange, include 10 aldehydes, 8 ketones, 22 alcohols, 12 esters, 11 hydrocarbons and 34 terpenes. D-limonene was shown to be the predominant compound in fresh navel oranges. The results show that comprehensive two-dimensional gas chromatography provided reliable and comprehensive information on the aroma compounds in fresh navel oranges.

中文翻译：

---

多维气相色谱四极杆飞行时间质谱法测定鲜脐橙中挥发性风味物质

摘要 新鲜水果的香气成分是评价品质和消费者接受度或抵制度的关键因素。由于当前分析方法的灵敏度有限，在大多数新鲜水果的研究中，许多重要的香气成分都没有被识别。本工作采用多维气相色谱四极杆飞行时间质谱法对鲜脐橙中的香气成分进行了研究，该技术可在一台仪器上同时实现一维和全面二维分离，无需更换色谱柱. 结果，综合二维气相色谱共测定了97种挥发性化合物，而一维气相色谱仅鉴定了44种化合物。确定的化合物，占 98。橙子中 30% 的挥发性化学物质包括 10 种醛、8 种酮、22 种醇、12 种酯、11 种烃和 34 种萜烯。D-柠檬烯被证明是新鲜脐橙中的主要化合物。结果表明，综合二维气相色谱法为新鲜脐橙中的香气化合物提供了可靠而全面的信息。