Abstract Trace detection of antibiotic ciprofloxacin by surface-enhanced Raman scattering (SERS) remains a challenge, because this compound is water-insoluble and amphoteric and it has no functional groups to combine with gold nanoparticles. Herein, ciprofloxacin was physically adsorbed on a self-assembly gold nanofilm for realizing quantitative SERS detection of ciprofloxacin in fish. A gold nanofilm containing ciprofloxacin spontaneously formed at the interface of gold colloids/n-hexane by vortex mixing in a few seconds without ethanol or other organic additives. The density of the gold nanoparticles on the interface which influences the SERS effect was controlled by varying the vortex mixing time and the volume of n-hexane. Furthermore, the proposed substrates with an enhancement factor of 2.7 × 106 were successfully applied for the determination of ciprofloxacin in fish samples with a linear range from 0.5 to 10.0 μg/mL and a detection limit of 0.19 μg/mL. The recovery measurements showed that the proposed SERS method had satisfactory precision and accuracy.

中文翻译：

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使用液-液自组装金纳米膜通过表面增强拉曼散射测定鱼类中的环丙沙星

摘要 通过表面增强拉曼散射 (SERS) 对抗生素环丙沙星进行痕量检测仍然是一个挑战，因为该化合物不溶于水且具有两性，并且没有与金纳米粒子结合的官能团。在此，环丙沙星物理吸附在自组装金纳米膜上，以实现鱼类中环丙沙星的定量 SERS 检测。在没有乙醇或其他有机添加剂的情况下，通过涡旋混合在几秒钟内在金胶体/正己烷的界面处自发形成含有环丙沙星的金纳米膜。通过改变涡旋混合时间和正己烷的体积来控制界面上影响 SERS 效应的金纳米粒子的密度。此外，所提出的基板的增强因子为 2。7 × 106 成功应用于鱼类样品中环丙沙星的测定，线性范围为 0.5 至 10.0 μg/mL，检出限为 0.19 μg/mL。回收率测量表明，所提出的 SERS 方法具有令人满意的精密度和准确度。