Fibronectin Bound to a Fibrous Substrate Has Chondrogenic Induction Properties.,Biomacromolecules

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Fibronectin Bound to a Fibrous Substrate Has Chondrogenic Induction Properties.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-01-31 , DOI: 10.1021/acs.biomac.9b01546
Marta R Casanova _{1,

2} , Rui L Reis _{1,

2,

3} , Albino Martins _{1,

2} , Nuno M Neves _{1,

2,

3}

Affiliation

Articular cartilage is an avascular tissue characterized by a dense and specific extracellular matrix (ECM). Fibronectin (FN) is a key constituent of the pericellular ECM, assembled into a fibrillar matrix through a cell-mediated process, being implicated in chondrogenic events. In this study, we evaluate the chondrogenic potential of FN bound at the surface of an electrospun nanofibrous mesh (NFM). For that, anti-FN antibody was immobilized at the surface of NFMs, capable of selectively bind endogenous FN (eFN) from blood plasma. The chondrogenic potential of bound eFN was further assessed by culturing human bone marrow-derived mesenchymal stem cells (hBM-MSCs) during 28 days, in basal growth medium. The biological results indicate that NFMs biofunctionalized with eFN were able to successfully induce the chondrogenesis of hBM-MSCs by the high expression of SOX9, Aggrecan, Collagen type II. Therefore, biofunctionalized nanofibrous comprising eFN significantly enhance the efficacy of a cartilage tissue engineering strategy.

中文翻译：

结合到纤维基质上的纤连蛋白具有软骨诱导特性。

关节软骨是一种血管组织，特征是致密而特异的细胞外基质（ECM）。纤连蛋白（FN）是细胞周围ECM的关键组成部分，通过细胞介导的过程组装成纤维状基质，与软骨形成事件有关。在这项研究中，我们评估了电纺纳米纤维网（NFM）表面上FN的成软骨潜力。为此，将抗FN抗体固定在NFM的表面，能够选择性结合血浆中的内源性FN（eFN）。通过在基础生长培养基中培养人骨髓来源的间充质干细胞（hBM-MSC）28天，进一步评估了结合的eFN的软骨形成潜力。生物学结果表明，用eFN生物功能化的NFM通过高表达SOX9，Aggrecan，II型胶原蛋白能够成功诱导hBM-MSC的软骨形成。因此，包含eFN的生物功能化纳米纤维可显着增强软骨组织工程策略的功效。

更新日期：2020-01-31

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