Bisphenol A (BPA) is one of the most widely used chemicals in plastic industry, which enters the human body through occupational and food contact. We studied the protein changes in rat cerebral cortex to evaluate the neurotoxicity of BPA. Twenty-four adult male rats were randomly selected and divided into four groups and each group respectively received 0, 0.5, 5 and 50 mg/kg of BPA for 4 weeks orally. To determine the oxidative status, reduced glutathione content and the level of malondialdehyde were measured in brain cortical tissue. The proteins of each sample extracted and separated on a two-dimensional acrylamide gel electrophoresis. From the obtained protein map, the 10 most altered protein spots were used for mass spectroscopy analysis. The lipid peroxidation in both doses of 0.5 and 5 mg/kg was significantly higher than the control group, but the glutathione content had no significant difference between the groups. Based on the results of the MS data analysis by the MASCOT database search engine, 10 proteins with altered intensity were identified as pyruvate kinase, alpha-enolase, aconitate hydratase, creatine kinase B-type, phosphatidylethanolamine-binding protein 1, 14-3-3 protein eta, guanine nucleotide-binding protein subunit beta-1, dihydropyrimidinase-related protein 2, glutamine synthetase and the neurofilament light polypeptide. There are several reports suggesting that the increase or decrease in the level and activity of these 10 proteins, similar to those observed in this study, is related to some neurological and psychosocial disorders including neurodegenerative diseases, schizophrenia, depression, epilepsy and some brain tumors.

中文翻译：

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暴露于双酚A的大鼠大脑皮质蛋白质谱的变化：蛋白质组学研究。

双酚A（BPA）是塑料工业中使用最广泛的化学物质之一，它通过职业和食品接触进入人体。我们研究了大鼠大脑皮质的蛋白质变化，以评估BPA的神经毒性。随机选择24只成年雄性大鼠，分为四组，每组分别口服0、0.5、5和50 mg / kg BPA，持续4周。为了确定氧化状态，在大脑皮层组织中测量了降低的谷胱甘肽含量和丙二醛水平。每个样品的蛋白质在二维丙烯酰胺凝胶电泳上提取并分离。从获得的蛋白质图谱中，将10个变化最大的蛋白质斑点用于质谱分析。0.5和5 mg / kg剂量下的脂质过氧化作用均显着高于对照组，但两组之间的谷胱甘肽含量没有显着差异。根据MASCOT数据库搜索引擎的MS数据分析结果，确定了强度发生变化的10种蛋白质，包括丙酮酸激酶，α-烯醇酶，乌头酸水合酶，肌酸激酶B型，磷脂酰乙醇胺结合蛋白1、14-3- 3蛋白eta，鸟嘌呤核苷酸结合蛋白β-1亚单位，二氢嘧啶酶相关蛋白2，谷氨酰胺合成酶和神经丝轻多肽。有几篇报道表明，这10种蛋白质的水平和活性的升高或降低，与本研究中观察到的相似，与某些神经系统和社会心理疾病有关，包括神经退行性疾病，精神分裂症，抑郁症，癫痫病和某些脑瘤。