Abstract Electrospinning of Gelatin (G) and Poly-3-hydroxybutyric acid (P) incorporated with anionic drug (AgSD) loaded hydrotalcite (L) (L-AgSD) is carried out to fabricate a nanofibrous scaffold which would recreate the native extracellular matrix suitable for cutaneous regeneration. The L-AgSD complex was augmented into electrospun nanofibers of diameter 100–140 nm. The physiochemical (XRD, FTIR), morphological (SEM), mechanical (tensile strength) and biological (in vitro and in vivo) properties of the developed wound construct were studied. Antimicrobial studies reveal the potential activity against microbial infection. Studies on drug release kinetics demonstrate a controlled release of 86% in 72 h. In vitro biocompatibility studies using NIH 3T3 fibroblast cell line showed excellent cell adhesion and cell proliferation indicating the biocompatible nature of the scaffold. The matrix accelerated wound healing on Pseudomonas infected burn wound induced on rat models. The tailored matrix is promising as an impending nanohybrid construct for burn wound injuries with controlled drug release and antibacterial activity.

中文翻译：

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耐用的纳米纤维基质添加了类水滑石化合物，用于烧伤创面的皮肤再生

摘要 明胶 (G) 和聚 3-羟基丁酸 (P) 与负载阴离子药物 (AgSD) 的水滑石 (L) (L-AgSD) 进行静电纺丝以制造纳米纤维支架，该支架将重建适合的天然细胞外基质。用于皮肤再生。L-AgSD 复合物被增强为直径 100-140 nm 的电纺纳米纤维。研究了所开发的伤口构造的物理化学（XRD、FTIR）、形态学（SEM）、机械（拉伸强度）和生物学（体外和体内）特性。抗微生物研究揭示了抗微生物感染的潜在活性。对药物释放动力学的研究表明，72 小时内的控释率为 86%。使用 NIH 3T3 成纤维细胞系进行的体外生物相容性研究显示出优异的细胞粘附和细胞增殖，表明支架的生物相容性。在大鼠模型上诱导的假单胞菌感染烧伤伤口上，基质加速伤口愈合。定制的基质有望作为即将到来的纳米混合结构，用于具有可控药物释放和抗菌活性的烧伤伤口。