Pseudomonas aeruginosa strain S3 exhibited high esterolytic activity against poly (lactic acid) (PLA) at environmental temperature (∼30 °C). A comprehensive study was conducted for optimization of physico-chemical parameters for high-throughput esterase production using Plackett–Burman Design and Central Composite Design. PLA degradation by Pseudomonas aeruginosa strain S3 was confirmed during preliminary studies using Fourier Transform Infrared Spectroscopy (FTIR) and scanning electron microscopy (SEM). The date obtained from statistical optimization demonstrated interactive action of inoculum size with peptone, yeast extract and Tween 20 was more influential towards esterase activity. The following medium constituents were optimized for maximum esterase production [(g/L) Glucose, 0.8; Peptone, 10.5; Yeast extract, 15; MgSO₄.7H₂O, 0.3; Sodium citrate, 4; CaCl₂, 1; NaCl, 1; FeSO₄.7H₂O, 1; Inoculum size 75 mL and Tween 20, 3.8 mL (v/v)]. The molecular weight of purified esterase was approximately 35 kDa with apparent K_m and V_max values, i.e., K_m 12.38 μM and V_max 769.23 U/mg, respectively. The enzyme showed stability over a broad range of pH (3–10) and temperature (20–40 °C). The activity of the purified enzyme was significantly enhanced by non-polar detergents (Tween 80 and Triton-X100), glycerol as well as both mono and divalent cations (Co⁺² and K⁺¹). Hydrolysis of PLA films with purified esterase released oligomers of medium chain length (n = 6–13), reaffirming the biodegradation potential of Pseudomonas aeruginosa strain S3 against PLA. The results obtained from the study demonstrate the potential of our strain and its enzymes in development of high efficient system of PLA biodegradation and recovery processes.

铜绿假单胞菌菌株S3在环境温度（〜30°C）下对聚乳酸（PLA）具有很高的酯分解活性。使用Plackett–Burman设计和中央复合设计，进行了一项综合研究，以优化用于高通量酯酶生产的理化参数。铜绿假单胞菌对PLA的降解在初步研究中，使用傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）确认了S3菌株。从统计优化中获得的数据表明，接种量与蛋白one，酵母提取物和吐温20的相互作用对酯酶活性具有更大的影响。优化了以下培养基成分以最大程度地提高酯酶的产量[（g / L）葡萄糖，0.8；蛋白ept10.5；酵母提取物15；MgSO ₄ .7H ₂ O，0.3；M ₊ 0.4。柠檬酸钠4; 氯化钙_2，1 ; 氯化钠1; FeSO ₄ .7H ₂ O，1; 接种量为75 mL，吐温20为3.8 mL（v / v）]。纯化的酯酶的分子量约为35 kDa，表观K _m和V _max值，即K _m 12.38μM和V _max 769.23 U / mg。该酶在很宽的pH（3-10）和温度（20-40°C）范围内都显示出稳定性。非极性去污剂（吐温80和Triton-X100），甘油以及单价和二价阳离子（Co ⁺²和K ⁺¹）均显着提高了纯化酶的活性。用纯化的酯酶水解PLA膜可释放中等链长（n = 6–13）的低聚物，从而重申了铜绿假单胞菌的生物降解潜力抗PLA的S3菌株。从研究中获得的结果证明了我们的菌株及其酶在开发高效PLA生物降解和回收过程系统方面的潜力。