An immunochromatographic test system for the determination of lincomycin in foodstuffs of animal origin.,Journal of Chromatography B

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An immunochromatographic test system for the determination of lincomycin in foodstuffs of animal origin.
Journal of Chromatography B ( IF 2.8 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.jchromb.2020.122014
Olga D Hendrickson ₁ , Elena A Zvereva ₁ , Demid S Popravko ₁ , Anatoly V Zherdev ₁ , Chuanlai Xu ₂ , Boris B Dzantiev ₁

Affiliation

The aim of this study was to develop a rapid and sensitive immunochromatographic test system for the detection of lincomycin (LIN), which belongs to the lincosamide group of antibiotics and contaminates food products of animal origin. Two formats of immunochromatographic analysis (ICA) based on different approaches of introducing gold nanoparticles (GNPs) as a label were compared. It was demonstrated that an indirect ICA method where GNPs were conjugated with anti-species antibodies allowed the achievement of both instrumental and visual detection limits of LIN almost two orders of magnitude lower than those achieved in the standard direct ICA format. In the optimized conditions, the developed indirect ICA allowed for the detection of LIN within 15 min, with instrumental and visual detection limits of 8 pg/mL and 0.8 ng/mL. The assay showed 40% cross-reactivity to clindamycin (CLIN) as a structural analogue of LIN, with no interaction with antibiotics from other classes. The developed ICA was applied for LIN detection in a panel of food products. No treatment of cow milk was necessary before the analysis. For chicken eggs and honey, a simple procedure of preliminary sample preparation was developed, which fully prevented a matrix influence on the assay results. It was demonstrated that ICA could detect LIN in food products while preserving the same analytical characteristics as in the buffer. The analytical recoveries of LIN in foodstuffs were 93.8-125% with coefficients of variations of 5.3-14.0%.

中文翻译：

一种用于动物源性食品中林可霉素测定的免疫色谱测试系统。

这项研究的目的是开发一种快速灵敏的免疫色谱测试系统，用于检测林可霉素（LIN），它属于抗生素的林可酰胺类，并污染了动物性食品。比较了两种基于引入金纳米颗粒（GNP）作为标记的不同方法的免疫色谱分析（ICA）格式。结果表明，间接ICA方法将GNP与抗物种抗体偶联，可以实现LIN的仪器和视觉检测极限，这比标准直接ICA格式所实现的极限低了近两个数量级。在优化的条件下，开发的间接ICA允许在15分钟内检测LIN，仪器和视觉检测极限分别为8 pg / mL和0.8 ng / mL。该测定显示与LIN的结构类似物克林霉素（CLIN）有40％的交叉反应性，与其他类别的抗生素没有相互作用。所开发的ICA被用于一系列食品中的LIN检测。分析前无需处理牛奶。对于鸡蛋和蜂蜜，开发了一种简单的样品前处理程序，可以完全防止基质对测定结果的影响。事实证明，ICA可以检测食品中的LIN，同时保留与缓冲液相同的分析特性。食品中LIN的分析回收率为93.8-125％，变异系数为5.3-14.0％。所开发的ICA被用于一系列食品中的LIN检测。分析前无需处理牛奶。对于鸡蛋和蜂蜜，开发了一种简单的样品前处理程序，可以完全防止基质对测定结果的影响。事实证明，ICA可以检测食品中的LIN，同时保留与缓冲液相同的分析特性。食品中LIN的分析回收率为93.8-125％，变异系数为5.3-14.0％。所开发的ICA被用于一系列食品中的LIN检测。分析前无需处理牛奶。对于鸡蛋和蜂蜜，开发了一种简单的样品前处理程序，可以完全防止基质对测定结果的影响。事实证明，ICA可以检测食品中的LIN，同时保留与缓冲液相同的分析特性。食品中LIN的分析回收率为93.8-125％，变异系数为5.3-14.0％。事实证明，ICA可以检测食品中的LIN，同时保留与缓冲液相同的分析特性。食品中LIN的分析回收率为93.8-125％，变异系数为5.3-14.0％。事实证明，ICA可以检测食品中的LIN，同时保留与缓冲液相同的分析特性。食品中LIN的分析回收率为93.8-125％，变异系数为5.3-14.0％。

更新日期：2020-02-03

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