A retrospective study of officially diagnosed poxvirus infections in cattle in Distrito Federal (DF), Brazil, between 2015 and 2018 was performed. All cases were investigated by the DF Official Veterinary Service. In the most cases, samples of oral, cutaneous (teats, udder) or foot lesions were submitted to molecular diagnosis by PCR. In approximately 70% of the cases, additional samples were also submitted for histopathology. Ninety‐three out of 2,467 clinically examined cattle (from 385 farms) presented suggestive and/or compatible lesions with poxviruses. Fifty‐two out of these 93 cases were confirmed as poxviruses: 27 vaccinia virus (VACV), 9 pseudocowpox virus (PCPV), 8 bovine papular stomatitis virus (BPSV), 5 coinfection by PCPV and BPSV and 3 unidentified parapoxvirus. The clinical cases were observed in farms with different exploration (beef, dairy or mixed) from 9 out of 30 administrative regions of DF. Gross findings consisted of papules, vesicles, ulcers, scabs and scars and varied of type, severity and affected tissue, according to the detected virus. A single human case was observed associated with a BPSV infection. Histologically, the lesions were very similar, independently of the detected poxvirus, and included mild to moderate, superficial, multifocal inflammatory infiltrate of lymphocytes, plasma cells, macrophages and/or neutrophils, with acanthosis and parakeratotic hyperkeratosis, usually associated with serous content, cellular debris and spongiosis. In the ulcerated lesions, there were focally extensive areas of necrosis with severe infiltrate of neutrophils in the adjacent connective tissue. Few to moderate amount of 4‐ to 8‐µm eosinophilic inclusion bodies were observed in the cytoplasm of keratinocytes in 6 cases (2 of VACV, 2 of PCPV and 2 of PCPV/BPSV coinfection). Data of the current study demonstrate the wide circulation of different poxviruses in cattle from DF.

中文翻译：

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从巴西Distrito Federal诊断为牛的痘病毒（2015-2018）。

在2015年至2018年之间，对巴西Distrito Federal（DF）的牛的官方诊断的痘病毒感染进行了回顾性研究。所有案件均由DF官方兽医服务进行了调查。在大多数情况下，口腔，皮肤（乳头，乳房）或足部病变的样本将通过PCR进行分子诊断。在大约70％的病例中，还提交了其他样本进行组织病理学检查。在2,467例经过临床检查的牛（来自385个农场）中，有93例表现出提示性和/或相容性痘病毒。在这93例病例中，有52例确诊为痘病毒：27株牛痘病毒（VACV），9例伪牛痘病毒（PCPV），8例牛丘疹性口炎病毒（BPSV），5例PCPV和BPSV合并感染以及3例未鉴定的副痘病毒。在DF的30个行政区域中有9个在具有不同勘探方式（牛肉，乳制品或混合饲料）的农场中观察到了临床病例。根据检测到的病毒，总体发现包括丘疹，囊泡，溃疡，结sc和疤痕，并且类型，严重性和受影响的组织各不相同。观察到与BPSV感染有关的单个人类病例。从组织学上看，病变非常相似，与所检测的痘病毒无关，包括淋巴细胞，浆细胞，巨噬细胞和/或嗜中性粒细胞的轻度至中度，浅表性，多灶性炎症浸润，伴棘皮病和角化不全角化，通常与浆液含量，细胞相关碎屑和海绵状病变。在溃疡性病变中，在邻近的结缔组织中存在广泛的坏死灶区域，嗜中性粒细胞严重浸润。6例角质形成细胞的细胞质中观察到很少至中等量的4至8 µm嗜酸性包涵体（VACV 2例，PCPV 2例，PCPV / BPSV合并2例）。当前研究的数据表明，不同痘病毒在DF牛中的广泛传播。