Capsular contracture remains a challenge in plastic surgery and represents one of the most common postoperative complications following alloplastic breast reconstruction. The impact of the surface structure of silicone implants on the foreign body reaction and the behaviour of connective tissue-producing cells has already been discussed. The aim of this study was to investigate different pore sizes of silicone surfaces and their influence on human fibroblasts in an in vitro model. Four different textures (no, fine, medium and coarse texture) produced with the salt-loss technique, have been assessed in an in vitro model. Human fibroblasts were seeded onto silicone sheets and evaluated after 1, 4 and 7 days microscopically, with viability assay and gene expression analysis. Comparing the growth behaviour and adhesion of the fibroblasts on the four different textures, a dense cell layer, good adhesion and bridge-building ability of the cells could be observed for the fine and medium texture. Cell number and viability of the cells were increasing during the time course of experiments on every texture. TGFß1 was lowest expressed on the fine and medium texture indicating a trend for decreased fibrotic activity. For silicone surfaces produced with the salt-loss technique, we were able to show an antifibrotic effect of smaller sized pores. These findings underline the hypothesis of a key role of the implant surface and the pore size and pore structure in preventing capsular contracture.

中文翻译：

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大小很重要-人成纤维细胞在具有不同孔径的网状有机硅表面上的体外行为。

囊膜挛缩症在整形外科中仍然是一个挑战，并且是同种异体乳房重建术后最常见的术后并发症之一。已经讨论了有机硅植入物的表面结构对异物反应和结缔组织产生细胞行为的影响。这项研究的目的是在体外模型中研究有机硅表面的不同孔径及其对人成纤维细胞的影响。已经在体外模型中评估了用盐损失技术产生的四种不同质地（无，精细，中等和粗糙质地）。将人类成纤维细胞接种到硅树脂片上，并在1、4、7天后进行显微镜评估，并进行活力测定和基因表达分析。比较成纤维细胞在四种不同质地上的生长行为和附着力，可以观察到细密和中等质地的细胞层致密，细胞具有良好的附着力和桥接能力。在每种质地的实验过程中，细胞数量和细胞活力都在增加。TGFβ1在细小和中等质地上最低表达，表明纤维化活性降低趋势。对于用盐分损失技术生产的有机硅表面，我们能够显示出较小尺寸的毛孔的抗纤维化作用。这些发现强调了假体的表面以及孔径和孔结构在防止包膜挛缩中起关键作用的假设。可以观察到细小和中等质地的细胞具有良好的粘附性和建桥能力。在每种质地的实验过程中，细胞数量和细胞活力都在增加。TGFβ1在细小和中等质地上最低表达，表明纤维化活性降低趋势。对于用盐分损失技术生产的有机硅表面，我们能够显示出较小尺寸的毛孔的抗纤维化作用。这些发现强调了假体的表面以及孔径和孔结构在防止包膜挛缩中起关键作用的假设。可以观察到细小和中等质地的细胞具有良好的粘附性和建桥能力。在每种质地的实验过程中，细胞数量和细胞活力都在增加。TGFβ1在细小和中等质地上最低表达，表明纤维化活性降低趋势。对于用盐分损失技术生产的有机硅表面，我们能够显示出较小尺寸的毛孔的抗纤维化作用。这些发现强调了假体的表面以及孔径和孔结构在防止包膜挛缩中起关键作用的假设。对于用盐分损失技术生产的有机硅表面，我们能够显示出较小尺寸的毛孔的抗纤维化作用。这些发现强调了假体的表面以及孔径和孔结构在防止包膜挛缩中起关键作用的假设。对于用盐分损失技术生产的有机硅表面，我们能够显示出较小尺寸的毛孔的抗纤维化作用。这些发现强调了假体的表面以及孔径和孔结构在防止包膜挛缩中起关键作用的假设。