In this study, Eisenia fetida was taken as the test organism and tetracycline was taken as the stress compound. The artificial soil test was conducted to study the utilization intensity of different carbon sources (the Biolog-microplate supplied) by microorganisms under different stress times and stress concentrations. The changes in the in vivo key enzymes activities of earthworms and oxidative stress indicators, such as malondialdehyde (MDA), were explored. The canonical correlation analysis method was the first used to establish a analysis-model to explore the relationship between the functional diversity of microbial community and the oxidative stress in earthworms in vivo under different stress times and concentrations. Research shows: 1) after tetracycline stress, in the earthworm, the CAT, POD, SOD, GPX were related to the microbes that use carbohydrate carbon sources; the GST and AChE were related to the microbes that use polymer carbon sources; the MDA was related to the microbes that use carbon sources: amino acid, carboxylic acid and phenolic acid. 2) Under low concentrations of tetracycline stress, there was no significant relationship between the functional diversity of the microbial communities and the effects of oxidative stress at this concentration. The high concentration of tetracycline can be utilized to screen probiotics that alleviate the effects of oxidative stress. 3) The utilization of carbon sources by microbial community in the earthworm after stress can be used as biomarker of ecotoxicology. It provides a basic theoretical for adding beneficial carbon sources to combat oxidative damage in vivo.

在这项研究中，以艾塞尼亚（Eisenia fetida）为受试生物，以四环素为胁迫化合物。进行了人工土壤试验，以研究微生物在不同胁迫时间和胁迫浓度下对不同碳源（由Biolog-microplate提供）的利用强度。探讨了the体内关键酶活性和丙二醛（MDA）等氧化应激指标的变化。典型的相关分析方法是第一个用于建立分析模型的模型，用于探索体内explore群落的功能多样性与氧化应激之间的关系。在不同的压力时间和浓度下。研究表明：1）在四环素胁迫后，worm中的CAT，POD，SOD，GPX与利用碳水化合物碳源的微生物有关；GST和AChE与使用聚合物碳源的微生物有关；MDA与使用碳源的微生物有关：氨基酸，羧酸和酚酸。2）在低浓度的四环素胁迫下，微生物群落的功能多样性与该浓度下的氧化胁迫作用之间没有显着的关系。高浓度的四环素可用于筛选可减轻氧化应激影响的益生菌。3）应激后the中微生物群落对碳源的利用可作为生态毒理学的生物标志物。