The grafting of laccase-catalysed butyl paraben (BPH) and p-coumaric acid (PCA) onto chitosan were performed using an eco-friendly procedure at 40 °C and with a phosphate buffer (100 mM, pH 6.5). Linear potentiometric titration, Fourier-transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA) were used to characterise the graft ratio, and structural changes in the BPH- and PCA-chitosans: their antioxidant and antibacterial activities were then analysed. The results showed that the graft ratios of BPH-chitosan and PCA-chitosan were 8.0% and 10.2% respectively. The FTIR showed an absorption decrease in amino groups and a new band appeared at 1637 cm⁻¹ corresponding to CN vibrations that were characteristic of imines due to Schiff base formation between the amino groups of chitosan and the phenolic hydroxyl of BPH or PCA. The thermal stability of chitosan derivatives was thus improved. Additionally, the antioxidant capacities of chitosan derivatives were improved, especially for the PCA-chitosan derivative (IC₅₀ were 2.069 mg/mL and 0.349 mg/mL for DPPH⁺ and ABTS⁺ scavenging, respectively): the samples also exhibited an increased activity against Escherichia coli, Salmonella, Bacillus subtilis and Staphylococcus aureus compared to untreated chitosan.

漆酶催化的对羟基苯甲酸丁酯（BPH）和对香豆酸（PCA）在壳聚糖上的接枝反应是在40°C的环境友好方法下进行的，并使用磷酸盐缓冲液（100 mM，pH 6.5）。使用线性电位滴定，傅立叶变换红外光谱（FT-IR）和热重分析（TGA）表征接枝率，并分析BPH-和PCA-壳聚糖的结构变化：然后分析其抗氧化和抗菌活性。结果表明，BPH-壳聚糖和PCA-壳聚糖的接枝率分别为8.0％和10.2％。FTIR显示氨基吸收减少，并且在1637 cm ^-1处出现一个新谱带，对应于C由于壳聚糖的氨基和BPH或PCA的酚羟基之间形成席夫碱，N振动是亚胺的特征。因此改善了壳聚糖衍生物的热稳定性。此外，壳聚糖衍生物的抗氧化能力得到了改善，尤其是对于PCA-壳聚糖衍生物（清除DPPH ⁺和ABTS ^+的IC ₅₀分别为2.069 mg / mL和0.349 mg / mL ）：样品还显示出增强的抗大肠杆菌，沙门氏菌，枯草芽孢杆菌和金黄色葡萄球菌与未处理的壳聚糖相比。