Abstract Bacterial cellulose has multiple applications in various industries such as food, biomedical, textile due to its uniqueness of being a better bio-compatible coating agent, binding material, etc. In this study, optimization of the culture medium for producing BC from Leifsonia soli was carried out by selecting different parameters. Five significant factors such as maltose, pH, incubation days, soy whey and calcium chloride were estimated through ANOVA based response surface methodology. Maximum cellulose production (5.97 g/L) was obtained where maltose 1 % (w/v) supplemented with 0.8 % (v/v) soy whey and calcium chloride 0.8 % (w/v) at pH 6.5 for 7 days of incubation. In addition, assurance of cellulose production from bacteria was done by using High-performance liquid chromatography analysis. Further, the structure and purity of obtained cellulose were examined by SEM and elemental analysis where it was observed that the sample holds the value of carbon 44.1 ± 0.20 % and hydrogen 6.2 ± 0.3 %, respectively. This study concludes that the addition of maltose and soy whey could be used as carbon, nitrogen sources and calcium chloride was used as an additive for the bacterial cellulose production compared to the Hestrin Schramm medium. In addition, the calculated water holding capacity of the sample was found to be 73 %.

中文翻译：

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Leifsonia sol 细菌纤维素产量的统计优化及其理化表征

摘要 细菌纤维素因其作为更好的生物相容性涂层剂、粘合材料等的独特性而在食品、生物医学、纺织等各个行业中具有多种应用。本研究优化了从 Leifsonia soli 生产 BC 的培养基。通过选择不同的参数进行。通过基于方差分析的响应面方法估计了五个重要因素，例如麦芽糖、pH、孵化天数、大豆乳清和氯化钙。获得最大纤维素产量 (5.97 g/L)，其中 1% (w/v) 麦芽糖补充 0.8% (v/v) 大豆乳清和 0.8% (w/v) 氯化钙，pH 6.5，孵育 7 天。此外，通过使用高效液相色谱分析来确保从细菌中生产纤维素。更多，所得纤维素的结构和纯度通过 SEM 和元素分析进行​​检查，其中观察到样品的碳值分别为 44.1 ± 0.20 % 和氢值 6.2 ± 0.3 %。该研究得出的结论是，与 Hestrin Schramm 培养基相比，麦芽糖和大豆乳清的添加可用作碳源、氮源，而氯化钙可用作细菌纤维素生产的添加剂。此外，经计算，样品的持水能力为 73%。与 Hestrin Schramm 培养基相比，氮源和氯化钙用作细菌纤维素生产的添加剂。此外，经计算，样品的持水能力为 73%。与 Hestrin Schramm 培养基相比，氮源和氯化钙用作细菌纤维素生产的添加剂。此外，经计算，样品的持水能力为 73%。