Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy.,Nature Biomedical Engineering

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Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy.
Nature Biomedical Engineering ( IF 26.8 ) Pub Date : 2020-02-03 , DOI: 10.1038/s41551-019-0510-4
Jonathan J S Rickard _{1,

2} , Valentina Di-Pietro _{3,

4} , David J Smith ₅ , David J Davies ₄ , Antonio Belli _{3,

4} , Pola Goldberg Oppenheimer _{1,

6}

Affiliation

Current technologies for the point-of-care diagnosis of traumatic brain injury (TBI) lack sensitivity, require specialist handling or involve complicated and costly procedures. Here, we report the development and testing of an optofluidic device for the rapid and label-free detection, via surface-enhanced Raman scattering (SERS), of picomolar concentrations of biomarkers for TBI in biofluids. The SERS-active substrate of the device consists of electrohydrodynamically fabricated submicrometre pillars covered with a plasmon-active nanometric gold layer, integrated in an optofluidic chip. We show that the device can detect N-acetylasparate in finger-prick blood samples from patients with TBI, and that the biomarker is released immediately from the central nervous system after TBI. The simplicity, sensitivity and robustness of SERS-integrated optofluidic technology might eventually help the triaging of TBI patients and assist clinical decision making at point-of-care settings.

中文翻译：

通过表面增强拉曼光谱快速光流体检测生物标志物用于颅脑损伤。

用于创伤性脑损伤（TBI）的即时诊断的当前技术缺乏敏感性，需要专家处理或涉及复杂且昂贵的程序。在这里，我们报告了通过表面增强拉曼散射（SERS）对皮流体中TBI生物标志物的皮摩尔浓度进行快速，无标记检测的光流体装置的开发和测试。该设备的SERS活性基质由电流体动力学制造的亚微米柱组成，该柱上覆盖了等离激元活性纳米金层，并集成在光流体芯片中。我们显示该设备可以从TBI患者的手指刺血样品中检测到N-乙酰天冬氨酸，并且TBI后中枢神经系统会立即释放生物标志物。简单，

更新日期：2020-02-03

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