Nostoc flagelliforme is well known for its strong ecological adaptability in inhabiting desert biological soil crusts. However, the mechanism of its recovery from quiescent to active state after prolonged dormancy remains poorly characterized. Especially how exoproteome be related to the adaptive strategies and participate in the microalgae-bacteria interaction. In the present work, we analysed the intra- and extra-cellular proteome of N. flagelliforme over a complete rehydration period both in sterilization and in natural condition for the first time. The protein expression profile for N. flagelliforme has more fluctuations during the first 1 h after wetting but been relatively steady after fully hydrated. According to the extracellular proteomic datasets, we found a dynamic secretion of various extracellular hydrolytic enzymes and membrane transport proteins, which were related to peptidoglycan digestion and nutrient exchange respectively. Two-hundred and thirteen differentially expressed proteins induced by sterilization also reflect variation in nutrient exchange and highlight symbiosis between N. flagelliforme and surrounding bacteria. We also identified 112 phosphopeptides and 217 phosphorylation site of 95 protein of hydrated N. flagelliforme. The time course datasets we present here will be a reference for understanding the molecular processes underlying N. flagelliforme resuscitation and its potential role in microbial community diversification and soil desertification control.

中文翻译：

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细胞内和细胞外蛋白质组的动态表达以及附生细菌对水合发鞭毛的影响。

鞭毛发菜（Nostoc鞭毛）因其在栖息于沙漠生物土壤结皮中的强大生态适应性而闻名。但是，长期休眠后其从静止状态恢复到活动状态的机制仍然不明确。特别是外蛋白质组如何与适应策略相关并参与微藻-细菌相互作用。在目前的工作中，我们首次在灭菌和自然条件下，在完整的补液期内分析了鞭毛猪笼草的细胞内和细胞外蛋白质组。鞭毛猪笼草的蛋白质表达谱在润湿后的最初1 h内波动更大，但在完全水合后相对稳定。根据细胞外蛋白质组学数据，我们发现各种细胞外水解酶和膜转运蛋白的动态分泌，分别与肽聚糖消化和营养交换有关。灭菌诱导的213个差异表达的蛋白质也反映了养分交换的变化，并突出了鞭毛猪笼草与周围细菌之间的共生关系。我们还鉴定了水合鞭毛虫95种蛋白的112个磷酸肽和217个磷酸化位点。我们在这里展示的时间过程数据集将为理解鞭毛猪笼草复苏的分子过程及其在微生物群落多样化和土壤荒漠化控制中的潜在作用提供参考。灭菌诱导的213个差异表达的蛋白质也反映了养分交换的变化，并突出了鞭毛猪笼草与周围细菌之间的共生关系。我们还确定了水合鞭毛虫95种蛋白的112个磷酸肽和217个磷酸化位点。我们在这里展示的时间过程数据集将为理解鞭毛猪笼草复苏的分子过程及其在微生物群落多样化和土壤荒漠化控制中的潜在作用提供参考。灭菌诱导的213个差异表达的蛋白质也反映了养分交换的变化，并突出了鞭毛猪笼草与周围细菌之间的共生关系。我们还确定了水合鞭毛虫95种蛋白的112个磷酸肽和217个磷酸化位点。我们在这里展示的时间过程数据集将为理解鞭毛猪笼草复苏的分子过程及其在微生物群落多样化和土壤荒漠化控制中的潜在作用提供参考。