Molecular imprinting was conducted on a thin polymeric membrane stacked on the self-assembled monolayer of a gold plate. The covalent bonding method was used in the molecular imprinting process. Cholesterol was used as a target molecule. Coating with poly(methyl methacrylate) was followed by hydrolysis and extraction of cholesterol. The remaining site was used to recognize cholesterol. Using the cholesterol-imprinted gold electrode as a working electrode, the cholesterol recognition ability was estimated. Various cholesterol analogs were used to obtain the comparative data to determine the imprinting and selectivity factors. The results showed that molecular similarity was important to enhance the recognition. However, the presence of a hydroxyl functional group played a key role in enhancing the molecular recognition, suggesting that molecular polarity and hydrophobicity are important factors.

中文翻译：

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自组装单层上胆固醇印迹系统的选择性

分子印迹是在堆叠在金板自组装单层上的薄聚合物膜上进行的。在分子印迹过程中使用了共价键合方法。胆固醇被用作目标分子。用聚（甲基丙烯酸甲酯）包衣，然后水解和提取胆固醇。剩余的位点用于识别胆固醇。使用胆固醇印迹金电极作为工作电极，估计胆固醇识别能力。使用各种胆固醇类似物来获得比较数据，以确定印记和选择性因素。结果表明，分子相似性对于增强识别很重要。然而，羟基官能团的存在在增强分子识别方面发挥了关键作用，