当前位置：
X-MOL 学术
›
Spinal Cord
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The incidence and etiology of sperm DNA fragmentation in the ejaculates of males with spinal cord injuries.
Spinal Cord ( IF 2.1 ) Pub Date : 2020-01-30 , DOI: 10.1038/s41393-020-0426-6 Eduardo Vargas-Baquero 1 , Stephen Johnston 2 , Antonio Sánchez-Ramos 3 , Angel Arévalo-Martín 1 , Richard Wilson 4 , Jaime Gosálvez 5
Spinal Cord ( IF 2.1 ) Pub Date : 2020-01-30 , DOI: 10.1038/s41393-020-0426-6 Eduardo Vargas-Baquero 1 , Stephen Johnston 2 , Antonio Sánchez-Ramos 3 , Angel Arévalo-Martín 1 , Richard Wilson 4 , Jaime Gosálvez 5
Affiliation
|
STUDY DESIGN
Retrospective descriptive study.
OBJECTIVES
To determine the incidence and probable etiology of sperm DNA fragmentation (SDF) in a sample of males with spinal cord injury (SCI).
SETTING
Hospital in Toledo, Spain; University-based Genetics laboratory in Madrid, Spain.
METHODS
Semen collected by vibro-stimulation from 27 males with various levels of spinal cord injury. Classical semen parameters, SDF, leukocytospermia and pro-oxidant capacity were assessed and compared with a cohort of normozoospermic fertile donors (n = 10).
RESULTS
Males with SCI presented with lower semen quality compared with normozoospermic donors with respect to progressive motility (p = 0.0002), SDF (p < 0.00005), pro-oxidant capacity (p = 0.0191) and leukocytospermia (p < 0.00005). Although there was no significant correlation between semen quality and time since the lesion occurred, the period of abstinence appeared to be positively correlated with SDF (r = 0.486; p = 0.041). When the semen parameters of males with SCI were categorized based on those with cervical and thoracic lesions, sperm concentration was higher for those with cervical damage (p = 0.0257). Males with complete lesions (AIS A) had ejaculates that were lower in progressive motility (p = 0.0040) than those with incomplete injuries (AIS B-D).
CONCLUSIONS
Ejaculates of males with SCI have excessively elevated SDF when compared with normozoospermic donors, which is likely to be associated with coincident high levels of leucocytospermia and pro-oxidant capacity. We propose that these phenomena are caused by the accumulation and degeneration of spermatozoa in the cauda epididymidis.
中文翻译：
男性脊髓损伤的射精中精子DNA断裂的发生率和病因学。
研究设计回顾性描述性研究。目的确定男性脊髓损伤（SCI）样本中精子DNA片段化（SDF）的发生率和可能的病因。西班牙托莱多的SETTING医院；位于西班牙马德里的大学遗传学实验室。方法通过振动刺激从27名不同程度的脊髓损伤的男性中收集精液。评估经典的精液参数，SDF，白细胞精子症和抗氧化剂能力，并与一组正常精子能育供体（n = 10）进行比较。结果在进行性运动（p = 0.0002），SDF（p <0.00005），促氧化剂能力（p = 0.0191）和白细胞精子症（p <0.00005）方面，患有SCI的男性精子质量较正常精子供体低。尽管自病变发生以来精液质量与时间之间无显着相关性，但禁欲时间似乎与SDF呈正相关（r = 0.486； p = 0.041）。当根据患有颈椎和胸部病变的男性对SCI男性的精液参数进行分类时，患有颈椎损伤的男性的精子浓度较高（p = 0.0257）。完全损伤（AIS A）的男性射精的进行性运动能力较不完全损伤（AIS BD）的男性低（p = 0.0040）。结论与正常精子供体相比，患有SCI的男性射精的SDF过度升高，这可能与白细胞精子率高和促氧化剂能力高同时发生有关。
更新日期：2020-01-30
中文翻译：
男性脊髓损伤的射精中精子DNA断裂的发生率和病因学。
研究设计回顾性描述性研究。目的确定男性脊髓损伤（SCI）样本中精子DNA片段化（SDF）的发生率和可能的病因。西班牙托莱多的SETTING医院；位于西班牙马德里的大学遗传学实验室。方法通过振动刺激从27名不同程度的脊髓损伤的男性中收集精液。评估经典的精液参数，SDF，白细胞精子症和抗氧化剂能力，并与一组正常精子能育供体（n = 10）进行比较。结果在进行性运动（p = 0.0002），SDF（p <0.00005），促氧化剂能力（p = 0.0191）和白细胞精子症（p <0.00005）方面，患有SCI的男性精子质量较正常精子供体低。尽管自病变发生以来精液质量与时间之间无显着相关性，但禁欲时间似乎与SDF呈正相关（r = 0.486； p = 0.041）。当根据患有颈椎和胸部病变的男性对SCI男性的精液参数进行分类时，患有颈椎损伤的男性的精子浓度较高（p = 0.0257）。完全损伤（AIS A）的男性射精的进行性运动能力较不完全损伤（AIS BD）的男性低（p = 0.0040）。结论与正常精子供体相比，患有SCI的男性射精的SDF过度升高，这可能与白细胞精子率高和促氧化剂能力高同时发生有关。
京公网安备 11010802027423号