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A label-free immunosensor for the sensitive detection of hepatitis B e antigen based on PdCu tripod functionalized porous graphene nanoenzymes.
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2020-01-30 , DOI: 10.1016/j.bioelechem.2020.107461
Zhaoling Tan 1 , Linlin Cao 2 , Xiuxian He 1 , Hui Dong 1 , Qing Liu 1 , Pingping Zhao 3 , Yueyun Li 1 , Daopeng Zhang 1 , Wanshan Ma 4
Affiliation  

Nanomaterials with enzyme properties possess excellent catalytic activity and stability. We prepared new nanoenzymes to construct a label-free electrochemical immunosensor for the detection of hepatitis B e antigen (HBe Ag). In this study, PdCu tripod (PdCu TP) functionalized porous graphene (PG) nanoenzymes (PdCu TPs/PG) were prepared through the in situ reduction of PdCu tripods onto porous graphene. The catalytic Michaelis-Menten kinetic parameters of PdCu TPs/PG are better than horseradish peroxidase (HRP) and show enhanced peroxidase-like activity. Therefore, we used PdCu TPs/PG to catalyse the electrochemically active matrix of H2O2 and generate the synergistically amplified current signal for the subsequent sensitive detection of HBe Ag. Due to the good conductivity, large specific surface area and synergistic amplification of PdCu TPs/PG, the quantitative detection of HBe Ag shows a detection limit of 20 fg·mL-1 and linear range from 60 fg·mL-1 to 100 ng·mL-1. During the detection of human serum samples, PdCu TPs/PG shows good accuracy based on the standard addition method and a comparison with an ELISA. The prepared immunosensors exhibiting good selectivity, stability and reproducibility provide an important basis for determining the prognosis of hepatitis B and show potential applications in medical applications.

中文翻译:

基于PdCu三脚架功能化的多孔石墨烯纳米酶的灵敏检测乙型肝炎e抗原的无标记免疫传感器。

具有酶性质的纳米材料具有出色的催化活性和稳定性。我们准备了新的纳米酶,以构建用于检测乙型肝炎e抗原(HBe Ag)的无标记电化学免疫传感器。在这项研究中,通过将PdCu三脚架原位还原到多孔石墨烯上,制备了PdCu三脚架(PdCu TP)功能化的多孔石墨烯(PG)纳米酶(PdCu TPs / PG)。PdCu TPs / PG的催化Michaelis-Menten动力学参数优于辣根过氧化物酶(HRP),并显示出增强的过氧化物酶样活性。因此,我们使用PdCu TPs / PG催化H2O2的电化学活性基质,并产生协同放大的电流信号,用于随后的HBe Ag灵敏检测。由于PdCu TPs / PG具有良好的导电性,大的比表面积和协同放大作用,对HBe Ag的定量检测显示最低检测限为20 fg·mL-1,线性范围为60 fg·mL-1至100 ng·mL-1。在检测人血清样品期间,基于标准添加方法并与ELISA比较,PdCu TPs / PG显示出良好的准确性。制备的具有良好的选择性,稳定性和可重复性的免疫传感器为确定乙型肝炎的预后提供了重要的依据,并在医学应用中显示出潜在的应用前景。
更新日期:2020-01-31
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