当前位置：
X-MOL 学术
›
J. Biophotonics
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Comparison between high definition FT-IR, Raman and AFM-IR for subcellular chemical imaging of cholesteryl esters in prostate cancer cells.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-02-13 , DOI: 10.1002/jbio.201960094 Maciej Roman 1 , Tomasz P Wrobel 1 , Czeslawa Paluszkiewicz 1 , Wojciech M Kwiatek 1
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-02-13 , DOI: 10.1002/jbio.201960094 Maciej Roman 1 , Tomasz P Wrobel 1 , Czeslawa Paluszkiewicz 1 , Wojciech M Kwiatek 1
Affiliation
|
The family of vibrational spectroscopic imaging techniques grows every few years and there is a need to compare and contrast new modalities with the better understood ones, especially in the case of demanding biological samples. Three vibrational spectroscopy techniques (high definition Fourier‐transform infrared [FT‐IR], Raman and atomic force microscopy infrared [AFM‐IR]) were applied for subcellular chemical imaging of cholesteryl esters in PC‐3 prostate cancer cells. The techniques were compared and contrasted in terms of image quality, spectral pattern and chemical information. All tested techniques were found to be useful in chemical imaging of cholesterol derivatives in cancer cells. The results obtained from FT‐IR and Raman imaging showed to be comparable, whereas those achieved from AFM‐IR study exhibited higher spectral heterogeneity. It confirms AFM‐IR method as a powerful tool in local chemical imaging of cells at the nanoscale level. Furthermore, due to polarization effect, p‐polarized AFM‐IR spectra showed strong enhancement of lipid bands when compared to FT‐IR.
中文翻译：
高清FT-IR，拉曼光谱和AFM-IR在前列腺癌细胞中胆甾醇酯亚细胞化学成像中的比较。
振动光谱成像技术的家庭每隔几年就在增长，因此有必要将新的模式与更好地理解的模式进行比较和对比，特别是在需要生物样品的情况下。三种振动光谱技术（高清傅立叶变换红外[FT-IR]，拉曼光谱和原子力显微镜红外[AFM-IR]）被用于PC-3前列腺癌细胞中胆固醇酯的亚细胞化学成像。在图像质量，光谱图和化学信息方面对这些技术进行了比较和对比。发现所有测试的技术可用于癌细胞中胆固醇衍生物的化学成像。从FT-IR和拉曼成像获得的结果具有可比性，而从AFM-IR研究获得的结果则显示出更高的光谱异质性。它证实了AFM‐IR方法是纳米级细胞局部化学成像的有力工具。此外，由于偏振效应，与FT-IR相比，p偏振AFM-IR光谱显示脂质带的增强。
更新日期：2020-02-13
中文翻译：
高清FT-IR，拉曼光谱和AFM-IR在前列腺癌细胞中胆甾醇酯亚细胞化学成像中的比较。
振动光谱成像技术的家庭每隔几年就在增长，因此有必要将新的模式与更好地理解的模式进行比较和对比，特别是在需要生物样品的情况下。三种振动光谱技术（高清傅立叶变换红外[FT-IR]，拉曼光谱和原子力显微镜红外[AFM-IR]）被用于PC-3前列腺癌细胞中胆固醇酯的亚细胞化学成像。在图像质量，光谱图和化学信息方面对这些技术进行了比较和对比。发现所有测试的技术可用于癌细胞中胆固醇衍生物的化学成像。从FT-IR和拉曼成像获得的结果具有可比性，而从AFM-IR研究获得的结果则显示出更高的光谱异质性。它证实了AFM‐IR方法是纳米级细胞局部化学成像的有力工具。此外，由于偏振效应，与FT-IR相比，p偏振AFM-IR光谱显示脂质带的增强。
京公网安备 11010802027423号