Multicomposite super-resolution microscopy: Enhanced Airyscan resolution with radial fluctuation and sample expansions.,Journal of Biophotonics

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Multicomposite super-resolution microscopy: Enhanced Airyscan resolution with radial fluctuation and sample expansions.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-02-12 , DOI: 10.1002/jbio.201960211
Baoju Wang ₁ , Longfang Yao ₁ , Yueyue Jing ₁ , Yiyan Fei ₁ , Qianming Bai _{2,

3} , Lan Mi ₁ , Jiong Ma _{1,

4,

5}

Affiliation

Either modulated illumination or temporal fluctuation analysis can assist super‐resolution techniques in overcoming the diffraction limit of conventional optical microscopy. As they are not contradictory to each other, an effective combination of spatial and temporal super‐resolution mechanisms would further improve the resolution of fluorescent images. Here, a super‐resolution imaging method called fluctuation‐enhanced Airyscan technology (FEAST) is proposed, which achieves ~40 nm lateral imaging resolution and is useful for a range of fluorescent proteins and organic dyes. It was demonstrated not only to obtain different subcellular super‐resolution images, but also to improve the accuracy of counting the average human epidermal growth factor receptor 2 (HER2) copy number for diagnosis in breast cancer. Furthermore, the combination of FEAST and sample expansion microscopy (Ex‐FEAST) improves the lateral resolution to ~26 nm.

中文翻译：

多重复合超分辨率显微镜：增强的Airyscan分辨率，具有径向波动和样品扩展。

调制照明或时间波动分析都可以帮助超分辨率技术克服常规光学显微镜的衍射极限。由于它们彼此并不矛盾，因此有效地组合空间和时间超分辨率机制将进一步提高荧光图像的分辨率。在此，提出了一种称为起伏增强Airyscan技术（FEAST）的超分辨率成像方法，该方法可实现约40 nm的横向成像分辨率，可用于多种荧光蛋白和有机染料。它被证明不仅可以获取不同的亚细胞超分辨率图像，而且还可以提高计算平均人类表皮生长因子受体2（HER2）拷贝数以诊断乳腺癌的准确性。此外，

更新日期：2020-02-12

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