Abstract This study explored the hydrolysis and fermentation of paper with hydrogen (H2) and volatile fatty acid (VFA) recovery using a natural microbial consortium added to lysimeters. Serial dilutions of rumen fluid in natura were performed to obtain a fermentative nonmethanogen consortium, which was used as inoculum in lysimeters under two different incubation temperatures, L1 at 35 °C and L2 at 55 °C. H2 was detected in both lysimeters: 791.5 mL (L1) and 70.4 mL (L2). In L1, the production of 16,856 mg L−1 acetic acid was likely due to the occurrence of homoacetogenesis. Under thermophilic conditions, L2, a low volume of H2 was detected, and the highlight was the production of ethanol and methanol at 2289 and 5614 mg L−1, respectively. According to the Shannon index, there was great diversity of microorganism populations in the rumen fluid in natura (6.34) compared with the purified rumen fluid (2.73), L1 (2.67) and L2 (3.21). The most abundant genus in the rumen fluid in natura was Prevotella (37.5 %); in the purified consortium (68.7 %) and L1 (63.8), Dysgonomonas; and in L2 (88.7 %), Thermicanus. In conclusion, the temperature influenced the microbial community structure, the metabolic route and, consequently, the main byproducts produced by the fermentative activity at 35 °C and 55 °C.

中文翻译：

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用中温和高温蒸煮仪发酵办公纸的瘤胃微生物高通量测序微生物群落分析

摘要 本研究使用添加到蒸渗仪中的天然微生物群落，探讨了利用氢气 (H2) 和挥发性脂肪酸 (VFA) 回收纸张的水解和发酵。在自然中进行瘤胃液的连续稀释以获得发酵的非产甲烷菌聚生体，在两种不同的培养温度下，L1 在 35 °C 和 L2 在 55 °C 下用作蒸渗仪中的接种物。在两种蒸渗仪中均检测到 H2：791.5 mL (L1) 和 70.4 mL (L2)。在 L1 中，16,856 mg L-1 乙酸的产生可能是由于同型乙酸的发生。在高温条件下，L2 检测到少量 H2，亮点是乙醇和甲醇的产量分别为 2289 和 5614 mg L-1。根据香农指数，与纯化的瘤胃液（2.73）、L1（2.67）和L2（3.21）相比，natura瘤胃液中微生物种群的多样性（6.34）差异很大。自然瘤胃液中含量最多的属是普氏菌属（37.5%）；在纯化的聚生体 (68.7 %) 和 L1 (63.8) 中，Dysgonomonas；在 L2 (88.7 %) 中，Thermicanus。总之，温度影响微生物群落结构、代谢途径，从而影响 35°C 和 55°C 发酵活动产生的主要副产物。