Alpha-cypermethrin (ACy) is a synthetic pyrethroid insecticide commonly used in agricultural practices for controlling a broad range of insect pests particularly belonging to the order Lepidoptera and Coleoptera. The present study aims to evaluate the toxic effect of ACy on microalgae by studying its influence on Chlorella sp. According to our knowledge, this is the first detailed study of ACy toxicity on microalgae. Significant growth inhibition of Chlorella sp. was observed at high ACy concentration (6−48 mg L⁻¹) during the entire 96 h bioassay. The 96 h median effective concentration (EC₅₀) of ACy was estimated to be 11.00 mg L⁻¹. Flow cytometry analysis showed an enhanced generation of reactive oxygen species (ROS) and intracellular lipid accumulation after 96 h exposure to 11.00 mg L^-1 of ACy. Further, the same ACy concentration showed a significant decrease in photosynthetic pigment content and an increase in antioxidant enzyme activity and malondialdehyde (MDA) content in Chlorella sp.

高效氯氰菊酯（ACy）是一种合成的拟除虫菊酯类杀虫剂，通常用于农业实践中，用于防治各种害虫，尤其是鳞翅目和鞘翅目。本研究旨在通过研究ACy对小球藻的影响来评估其对微藻的毒性作用。据我们所知，这是ACy对微藻毒性的第一个详细研究。小球藻的显着生长抑制。在整个96小时的生物测定过程中，均以高ACy浓度（6-48 mg L ^-1）观察到了这一现象。ACy的96小时平均有效浓度（EC ₅₀）估计为11.00 mg L ^-1。流式细胞仪分析显示，暴露于11.00 mg L ^-1的ACy 96小时后，活性氧（ROS）的产生和细胞内脂质的积累增加。此外，相同的ACY浓度表明在光合色素含量显著减少和增加抗氧化酶活性和丙二醛（MDA）的含量小球藻属。