Abstract Cultivating microalgae with wastewater offers a dual-beneficial strategy for the removal of nutrients coupled with the accumulation of valuable bioproducts. However, the dynamic mass flow in heterotrophic algal growth is far less known than autotrophic mode. This study particularly provided a new insight into this process by modelling heterotrophic Chlorella sorokiniana. A consensus model was proposed to describe the temporal change of (i) the carbon, nitrogen, and phosphorus in water, (ii) the algal biomass, (iii) the intracellular carbohydrate and lipid, and their correlation under various conditions simulating the complex wastewater environment. The dynamics for the decrement of ambient carbon and nutrients and the increment of biomass, intracellular carbohydrate and lipid was correlated for the first time. The model was calibrated and validated with experimental data of C. sorokiniana. It therefore provides a theoretical basis for the future scale-up of heterotrophic microalgae culture with complicated real wastewaters.

中文翻译：

---

废水中异养微藻培养的动力学模型：储存分子的产生和污染物的缓解

摘要 用废水培养微藻为去除营养物和积累有价值的生物产品提供了一种双重有益的策略。然而，异养藻类生长中的动态质量流远不如自养模式为人所知。这项研究通过对异养小球藻进行建模，特别为这一过程提供了新的见解。提出了一个共识模型来描述（i）水中的碳、氮和磷，（ii）藻类生物量，（iii）细胞内碳水化合物和脂质的时间变化，以及它们在模拟复杂废水的各种条件下的相关性环境。首次将环境碳和养分减少与生物量、细胞内碳水化合物和脂质增加的动态相关联。该模型使用 C. sorokiniana 的实验数据进行校准和验证。因此，它为未来用复杂的实际废水扩大异养微藻培养提供了理论基础。