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Platelet lysate-loaded PLGA nanoparticles in a thermo-responsive hydrogel intended for the treatment of wounds.
European Journal of Pharmaceutical Sciences ( IF 4.3 ) Pub Date : 2020-01-31 , DOI: 10.1016/j.ejps.2020.105231 Sergio Alberto Bernal-Chávez 1 , Sergio Alcalá-Alcalá 2 , Doris Cerecedo 3 , Adriana Ganem-Rondero 1
European Journal of Pharmaceutical Sciences ( IF 4.3 ) Pub Date : 2020-01-31 , DOI: 10.1016/j.ejps.2020.105231 Sergio Alberto Bernal-Chávez 1 , Sergio Alcalá-Alcalá 2 , Doris Cerecedo 3 , Adriana Ganem-Rondero 1
Affiliation
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A thermo-responsive hydrogel of Pluronic F-127, containing PLGA nanoparticles loaded with a platelet lysate for wound treatment, was prepared. A high rate of incorporation of the lysate (about 80%) in the nanoparticles was achieved by the double emulsion-solvent evaporation method. The nanoparticles were characterized by measuring their size (about 318 nm), polydispersity index (0.29) and Z potential (-17.6), as well as by infrared and calorimetric techniques, and determining their stability as a function of time. It was found through measures of transepidermal water loss that the hydrogel containing the nanoparticles was capable of providing a semi-occlusive environment, necessary for the recovery of a wound. The inclusion of lysate in nanoparticles and this in turn in the hydrogel allowed a gradual release, which would avoid contact of the total dose with the biological medium. Studies with fibroblasts and in vivo in mice showed that the hydrogel containing nanoparticles with platelet lysate promoted faster tissue regeneration than the lysate in its free form, so this system is presented as a good alternative for the treatment of wounds.
中文翻译：
热响应水凝胶中加载血小板裂解物的PLGA纳米颗粒，旨在治疗伤口。
制备了Pluronic F-127的热响应水凝胶，该凝胶包含PLGA纳米颗粒，该PLGA纳米颗粒负载有用于伤口处理的血小板裂解物。通过双重乳液-溶剂蒸发法实现了裂解物在纳米颗粒中的高掺入率（约80％）。通过测量纳米粒子的尺寸（约318 nm），多分散指数（0.29）和Z电位（-17.6）以及红外和量热技术，并确定其随时间的变化，对纳米粒子进行表征。通过表皮水分流失的测量发现，含有纳米颗粒的水凝胶能够提供半封闭环境，这是伤口恢复所必需的。溶胞产物包含在纳米颗粒中，并随后在水凝胶中允许逐渐释放，这样可以避免总剂量与生物介质接触。用成纤维细胞和小鼠体内进行的研究表明，与游离形式的裂解物相比，含有具有血小板裂解物的纳米颗粒的水凝胶促进组织再生更快，因此，该系统被认为是治疗伤口的良好选择。
更新日期：2020-01-31
中文翻译：
热响应水凝胶中加载血小板裂解物的PLGA纳米颗粒，旨在治疗伤口。
制备了Pluronic F-127的热响应水凝胶，该凝胶包含PLGA纳米颗粒，该PLGA纳米颗粒负载有用于伤口处理的血小板裂解物。通过双重乳液-溶剂蒸发法实现了裂解物在纳米颗粒中的高掺入率（约80％）。通过测量纳米粒子的尺寸（约318 nm），多分散指数（0.29）和Z电位（-17.6）以及红外和量热技术，并确定其随时间的变化，对纳米粒子进行表征。通过表皮水分流失的测量发现，含有纳米颗粒的水凝胶能够提供半封闭环境，这是伤口恢复所必需的。溶胞产物包含在纳米颗粒中，并随后在水凝胶中允许逐渐释放，这样可以避免总剂量与生物介质接触。用成纤维细胞和小鼠体内进行的研究表明，与游离形式的裂解物相比，含有具有血小板裂解物的纳米颗粒的水凝胶促进组织再生更快，因此，该系统被认为是治疗伤口的良好选择。
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