Eukaryotic genomes are packaged into highly condensed chromatin and this repressive chromatin barrier can be overcome by altering the chromatin structure via histone modification enzymes. Here, we report Wdr70 in Schizosaccharomyces pombe (spWdr70) plays important roles in multiple cellular processes including cell cycle progression, chromatin structure and DNA repair. Depletion of Wdr70 gene causes cell cycle delay, hypersensitivity to DNA damage reagents and quick phenotypic changes. Moreover, we observed strong genetic interaction between Wdr70 and genes regulating checkpoint and homologous recombination (HR), pinpointing the function of Wdr70 to DNA end resection. Finally, we show that the function of Wdr70 could be attributed to monoubiquitination of histone H2B (uH2B) in the vicinity of DNA double strand breaks (DSBs). Taken together, our data reveal that Wdr70 and H2B monoubiquitination-dependent chromatin modulation is required for chromatin homeostasis and genetic stability.

中文翻译：

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Wdr70调节裂变酵母中的组蛋白修饰和基因组维持。

真核生物基因组被包装到高度浓缩的染色质中，这种抑制性染色质屏障可以通过组蛋白修饰酶改变染色质结构来克服。在这里，我们报道了裂殖酵母中的Wdr70（spWdr70）在包括细胞周期进程，染色质结构和DNA修复在内的多个细胞过程中起着重要作用。Wdr70基因的消耗会导致细胞周期延迟，对DNA损伤试剂的超敏性和快速的表型变化。此外，我们观察到Wdr70与调节检查点和同源重组（HR）的基因之间有很强的遗传相互作用，从而明确了Wdr70在DNA末端切除中的功能。最后，我们表明Wdr70的功能可以归因于DNA双链断裂（DSBs）附近的组蛋白H2B（uH2B）的单泛素化。在一起