Breeding of high-lipid content microalgae in municipal wastewater is critical for large-scale microalgal biodiesel production. In this study, a strain of biodiesel promising microalga Scenedesmus obliquus with good stain resistance was obtained by N⁺ ion implantation, which was named as S1-A3. In the selenite enrichment (SE) medium, the lipid content of S1-A3 was up to 46.92%, which was increased by 24.1% than the original strain. Meanwhile, after 7 days of cultivation, the removal rates of TP,TN, NH₄⁺-N and COD in wastewater attained 95.72%, 80.30%, 87.25% and 85.43% by S1-A3, respectively. S1-A3 had a good genetic stability in both municipal wastewater and SE medium, and its lipid production capacity was improved after four successive cultivation in wastewater. RAPD analysis showed that the mutant and the original strain were interspecific variations. Therefore, N⁺ ion implantation was proved to be an approach that can significantly improve the lipid content and adaptability of Scenedesmus obliquus.

市政废水中高脂类微藻的育种对于大规模微藻生物柴油生产至关重要。在这项研究中，通过N ⁺离子注入获得了具有良好抗污性的有希望的微藻斜生藻生物柴油菌株，其被命名为S1-A3。在亚硒酸盐富集（SE）培养基中，S1-A3的脂质含量高达46.92％，比原始菌株增加了24.1％。同时，培养7天后，TP，TN，NH ₄ ⁺的去除率S1-A3处理废水中的-N和COD分别达到95.72％，80.30％，87.25％和85.43％。S1-A3在城市废水和SE培养基中均具有良好的遗传稳定性，在废水中连续培养四次后其脂质生产能力得到提高。RAPD分析表明该突变体和原始菌株是种间变异。因此，N ⁺离子注入被证明是一种可以显着提高斜生场面藻脂质含量和适应性的方法。