Mechanical disruption of lignocellulose during fermentation, cotreatment, is a nascent approach to increase biologically-mediated carbohydrate solubilization without exposure to high temperatures or chemicals. However, ability to tolerate the presence of milling at intensities sufficient to allow high lignocellulose solubilization yields has to date only been evaluated for Clostridium thermocellum and Saccharomyces cerevisiae, and demonstration of high carbohydrate solubilization has only been reported for switchgrass at the time of submission. Continuous ball milling during fermentation by C. thermocellum was found to be sufficient to allow high (>85%) total carbohydrate solubilization of corn stover and poplar as well as switchgrass. Under the conditions tested, ball milling had no apparent effect on soluble sugar fermentation by Escherichia coli and Thermoanaerobacterium saccharolyticum but completely inhibited carbohydrate fermentation by Zymomonas mobilis and Bacillus subtilis. Our results are consistent with the stress of milling being sufficient to overwhelm the inefficient fermentative metabolism of Z. mobilis and B. subtilis, but not to the other microorganisms tested in this work for which fermentation is more efficient and robust.

在发酵，共处理过程中，木质纤维素的机械破坏是一种新兴的方法，可以提高生物介导的碳水化合物的溶解度，而无需暴露于高温或化学药品中。然而，迄今为止，仅对于热纤梭菌和酿酒酵母评估了耐受足以允许高木质纤维素增产的强度的碾磨的能力，并且仅在提交时报道了柳枝switch具有高碳水化合物增溶的证明。热纤梭菌发酵过程中的连续球磨被发现足以使玉米秸秆和杨树以及柳枝switch的碳水化合物总量增高（> 85％）。在测试的条件下，球磨对大肠杆菌和解热厌氧杆菌的可溶性糖发酵没有明显影响，但完全抑制了运动发酵单胞菌和枯草芽孢杆菌对碳水化合物的发酵。我们的结果与碾磨的压力足以压倒运动发酵单胞菌和枯草芽孢杆菌的低效率发酵代谢相吻合，但与这项工作中测试的其他微生物相比，后者的发酵效率更高且更稳定。