Even though Hepatozoon spp. has been molecularly detected in several wild animals in Brazil, there is no report on the occurrence of Hepatozoon spp. DNA in bats in Brazil. This study aimed at detecting Hepatozoon, in addition to ectoparasites, in non-hematophagous bats sampled in central-western Brazil using blood smears, hematoxylin-eosin (HE)-staining liver/spleen preparations and molecular and phylogenetic techniques. A total of 135 spleen, 127 liver, and 133 blood samples were collected from 135 non-hematophagous bats from 12 different species in two different sites in Campo Grande, Mato Grosso do Sul state, in the Brazilian Cerrado region. Spleen and blood DNA samples were submitted to two conventional PCR protocols for Hepatozoon spp. based on 18S rRNA. No Hepatozoon spp. gamonts or meronts were observed in blood smears and HE-stained-liver preparations, respectively. While none of the spleen samples was positive for Hepatozoon spp. in the PCR assays, 5 (3 %) blood samples contained 18S rRNA Hepatozoon DNA, including 2/37 (5 %) Artibeus lituratus, 2/32 (6 %) A. planirostris, and 1/23 (4 %) Platyrrhinus lineatus. Out of 5 bats positive for Hepatozoon spp., 3 were parasitized by either Macronyssidae/Spinturnicidae mites or Streblidae flies. BLAST analysis showed that the sequences detected in bats had >99 % identity with Hepatozoon sequences detected in amphibians and reptiles from Brazil, including Hepatozoon caimani detected in Caiman crocodilus. The phylogenetic inferences estimated by the Maximum Likelihood and Bayesian methods clustered the Hepatozoon sequences detected in Brazilian bats with those detected in reptiles and amphibians.

即使Hepatozoon spp。在巴西的几种野生动物中已通过分子检测发现，尚无关于Hepatozoon spp发生的报道。巴西蝙蝠中的DNA。这项研究旨在利用血液涂片，苏木精-伊红（HE）染色肝/脾制剂以及分子和系统发育技术，在巴西中西部采样的非嗜血蝙蝠中检测除肝寄生虫外的肝虫。在巴西塞拉多州南马托格罗索州坎波格兰德的两个不同地点，从来自12个不同物种的135个非食噬性蝙蝠中收集了135个脾脏，127个肝脏和133个血液样本。将脾脏和血液DNA样本提交给Hepatozoon的两种常规PCR方案spp。基于18S rRNA。没有Hepatozoon物种。在血液涂片和HE染色肝制剂中分别观察到gamonts或melont。虽然没有一个脾脏样本对肝He菌呈阳性。在PCR测定中，5（3％）的血液样品中含有18S rRNA基因Hepatozoon DNA，包括2/37（5％）美洲果蝠属lituratus，2/32（6％）A. planirostris，和1/23（4％）白线蝠属绦虫。在对Hepatozoon spp。呈阳性的5只蝙蝠中，有3只被巨ny科/自旋科螨或Streblidae寄生。BLAST分析表明，在蝙蝠中检测到的序列与Hepatozoon的同源性> 99％在来自巴西的两栖动物和爬行动物中检测到的序列，包括在凯门鳄中检测到的Hepatozoon caimani。通过最大似然法和贝叶斯方法估计的系统发育推断将在巴西蝙蝠中检测到的Hepatozoon序列与在爬行动物和两栖动物中检测到的序列进行了聚类。