A toxicity test based on inhibition of reproduction in the green macroalga Ulva pertusa involves quantifying the change in thallus color as reproduction progresses. However, interpretation of this color change is reliant on the skill level of the examiner. This study aimed to validate a new toxicity test based on inhibition of reproduction in the green macroalga U. pertusa using a vital stain and programmed semi-automated analysis (using Image J) of the change in thallus color. The toxicity rank by inverse EC50 values was: irgarol (0.048 mg L-1) > Ag (0.132 mg L-1) > As (0.172 mg L-1) > simazine (0.378 mg L-1) > formaldehyde (0.442 mg L-1) > DCOIT (0.783 mg L-1) > ZnPT (3.556 mg L-1) > medetomidine (11.600 mg L-1) > phenol (29.316 mg L-1) > methanol (2,736 mg L-1) > ethanol (3,306 mg L-1). The sensitivity of the U. pertusa test to stream waters was similar to or lower than those of the commonly-used Lemna minor and Daphnia magna bioassays. The U. pertusa bioassay is sensitive to, and suitable for, testing various toxicants including metals, volatile organic compounds, herbicide, antifouling agents and phenol and can also be applied to testing freshwater quality after salinity adjustment.

中文翻译：

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程序化的半自动化百日草生物测定法在单一毒物和溪流水质测试中的应用。

基于抑制绿色大型藻类百日草繁殖的毒性试验涉及量化繁殖过程中th体颜色的变化。但是，这种颜色变化的解释取决于检查员的技术水平。这项研究旨在验证一种新的毒性试验，该试验基于使用活体染色和对th虫颜色变化的程序化半自动分析（使用图像J），抑制了绿色巨藻美国百日草的繁殖。EC50值反向的毒性排名是：依加洛尔（0.048 mg L-1）>银（0.132 mg L-1）>砷（0.172 mg L-1）>辛嗪（0.378 mg L-1）>甲醛（0.442 mg L -1）> DCOIT（0.783 mg L-1）> ZnPT（3.556 mg L-1）>美托咪定（11.600 mg L-1）>苯酚（29.316 mg L-1）>甲醇（2,736 mg L-1）>乙醇（3,306毫克L-1）。美国的敏感性 流水的百日咳试验与常用的Lemna minor和Daphnia magna生物测定法相似或更低。百日草生物测定法对各种有毒物质（包括金属，挥发性有机化合物，除草剂，防污剂和苯酚）敏感并且适用于测试，也可以用于调节盐度后的淡水质量。