Abstract The goal of this study is to construct bone scaffolds with neuroregulatory function. The scaffolds were prepared using bioglass (BG) powder, collagen (COL) solution, phosphatidylserine (PS) and ophiopogonin loaded COL microspheres as the main components. Combinatorial processing techniques involving chemical-crosslinking and freeze-drying were used. The resultant scaffold constructs were characterized in terms of their morphology, drug release kinetics, pharmacological and biological activities for osteoblast and neurocyte. The microporphology observation showed that the scaffolds had highly interconnected pores, favorable drug release kinetics in view of low initial release and slow average release rate. Moreover, cell studies showed that the involvement of ophiopogonin contributed to the proliferation and mineralization of MC3T3-E1 cells, and the neurite outgrowth of neuron. The drug-loaded composite scaffolds may therefore be a potential replacement in bone-tissue engineering.

中文翻译：

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生物活性麦冬素释放生物玻璃-胶原-磷脂酰丝氨酸支架以增强体外骨修复

摘要 本研究的目的是构建具有神经调节功能的骨支架。以生物玻璃（BG）粉、胶原蛋白（COL）溶液、磷脂酰丝氨酸（PS）和麦冬素负载的COL微球为主要成分制备支架。使用了涉及化学交联和冷冻干燥的组合加工技术。所得支架结构的特征在于它们的形态、药物释放动力学、成骨细胞和神经细胞的药理和生物活性。微观形态观察表明，支架具有高度互连的孔隙，鉴于低初始释放和缓慢的平均释放速率，有利于药物释放动力学。而且，细胞研究表明麦冬素的参与促进了MC3T3-E1细胞的增殖和矿化，以及神经元的轴突生长。因此，载药复合支架可能是骨组织工程的潜在替代品。