Dysregulation of microRNAs (miRNAs) is acknowledged in human cutaneous squamous cell carcinoma (cSCC). We hereby evaluated the ability of miRNA-216b (miR-216b) to impact human cSCC. cSCC tissues with corresponding adjacent normal tissues were collected from 40 patients diagnosed with cSCC where the expression pattern of miR-216b and targeting protein for Xenopus kinesin-like protein 2 (TPX2) was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blot analysis. A431 cells were transfected with miR-216b mimic, miR-216b inhibitor, or short interfering RNA against TPX2 to evaluate cell proliferation, invasion, migration, and apoptosis using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, scratch test, Transwell assay, and flow cytometry. TPX2 was highly expressed in cSCC tissues while miR-216b was poorly expressed in association with tumor differentiation, lymph node metastasis, and tumor node metastasis staging in patients with cSCC. In response to overexpressed miR-216b or silenced TPX2, cSCC cell proliferation, invasion, and migration were suppressed and apoptosis was stimulated, along with activated p53 signaling. Thus, upregulated miR-216b was capable of promoting apoptosis and inhibiting proliferation, invasion, and migration of cSCC cells by downregulating TPX2 through activation of the p53 signaling, highlighting a novel biomarker for novel treatment modalities against cSCC.

在人类皮肤鳞状细胞癌 (cSCC) 中确认了 microRNA (miRNA) 的失调。我们在此评估了 miRNA-216b (miR-216b) 影响人类 cSCC 的能力。从 40 名诊断为 cSCC 的患者中收集 cSCC 组织和相应的相邻正常组织，其中 miR-216b 的表达模式和非洲爪蟾的靶向蛋白驱动蛋白样蛋白 2 (TPX2) 通过定量逆转录酶聚合酶链反应 (qRT-PCR) 和蛋白质印迹分析确定。用 miR-216b 模拟物、miR-216b 抑制剂或针对 TPX2 的短干扰 RNA 转染 A431 细胞，以使用 3-(4,5-dimethylthiazol-2-yl)-2,5 评估细胞增殖、侵袭、迁移和凋亡-溴化二苯基四唑 (MTT) 检测、划痕检测、Transwell 检测和流式细胞术。TPX2 在 cSCC 组织中高表达，而 miR-216b 在 cSCC 患者中与肿瘤分化、淋巴结转移和肿瘤淋巴结转移分期相关的表达低。作为对过表达的 miR-216b 或沉默的 TPX2 的反应，cSCC 细胞增殖、侵袭和迁移受到抑制，细胞凋亡受到刺激，同时 p53 信号传导也被激活。因此，