Caveolin proteins electrochemical oxidation and interaction with cholesterol.,Bioelectrochemistry

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Caveolin proteins electrochemical oxidation and interaction with cholesterol.
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2020-01-29 , DOI: 10.1016/j.bioelechem.2019.107451
Isabel P G Fernandes ₁ , Ana Maria Oliveira-Brett ₁

Affiliation

Caveolae consist in lipid raft domains composed of caveolin proteins, cholesterol, glycosphingolipids, and GPI-anchored proteins. Caveolin proteins present three different types, caveolin 1 (CAV-1), caveolin 2 (CAV-2) and caveolin 3 (CAV-3), with a very similar structure and amino acid composition. The native caveolin proteins oxidation mechanism was investigated for the first time, at a glassy carbon electrode, using cyclic, square wave and differential pulse voltammetry. The three native caveolin proteins oxidation mechanism presented only one tyrosine and tryptophan amino acid residues oxidation peak. Denatured caveolin proteins presented also the tyrosine, tryptophan and cysteine amino acid residues oxidation peaks. The reverse cholesterol transport is related to caveolae and caveolin proteins, and CAV-1 is directly connected to cholesterol transport. The influence of cholesterol on the three caveolin proteins electrochemical behaviour was evaluated. In the absence and in the presence of cholesterol, significant differences in the CAV-1 oxidation peak current were observed.

中文翻译：

洞穴蛋白蛋白质电化学氧化并与胆固醇相互作用。

小窝包括由小窝蛋白，胆固醇，鞘糖脂和GPI锚定蛋白组成的脂筏结构域。小窝蛋白呈现三种不同类型，小窝蛋白1（CAV-1），小窝蛋白2（CAV-2）和小窝蛋白3（CAV-3），其结构和氨基酸组成非常相似。使用循环碳，方波和微分脉冲伏安法，首次在玻碳电极上研究了天然小窝蛋白的氧化机理。三种天然小窝蛋白的氧化机理仅呈现一个酪氨酸和色氨酸氨基酸残基的氧化峰。变性的窖蛋白还显示出酪氨酸，色氨酸和半胱氨酸氨基酸残基的氧化峰。胆固醇的反向转运与小窝蛋白和小窝蛋白有关，CAV-1与胆固醇的运输直接相关。评估胆固醇对三种小孔蛋白的电化学行为的影响。在不存在和存在胆固醇的情况下，观察到CAV-1氧化峰值电流的显着差异。

更新日期：2020-01-30

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