We studied the early protein profile in the ocular tissue extracted after LASIK and SMILE surgery. SMILE and LASIK was performed in contralateral eyes and stromal tissue samples were collected from 10 eyes of 5 donors. The stromal tissue samples were analyzed using label free quantification approach and ITRAQ labelling approach in LC-MS/MS. Combined functional analysis revealed many differentially expressed proteins which were involved in important biological processes. About 117 unique differentially expressed proteins were identified using two different proteomic approaches. Collagens, proteoglycans, corneal crystallins were enriched and showed differential expression in SMILE and LASIK as compared to the non-surgical control. Apart from these, 14-3-3 class of proteins, Lysozyme (LYZ), Macrophage Migratory Inhibitory Factor protein (MIF), Pigment Epithelial Derived Factor (PEDF) were differentially expressed when compared between LASIK and SMILE. Peroxiredoxin 1 (PRDX1) expression was found to be reduced in LASIK as compared to SMILE. The expression of Lysozyme C and Macrophage Migratory Inhibitory Factor inflammatory response was found to be less in SMILE as compared to LASIK. Western blot validation of specific markers such as Collagen IV (COL4), Keratocan (KERA), Lumican (LUM), Aldehyde dehydrogenase 3 A1 (ALDH3A1), Lysozyme C (LYZC) confirmed the differences in the protein levels observed in SMILE and LASIK operated tissues as compared to non-surgical controls. In conclusion, this study revealed the early molecular changes occurring in the cornea resulting from these two surgical procedures which may have implications on managing post-operative complications.

中文翻译：

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SMILE和LASIK手术后眼组织的早期生物学反应。

我们研究了LASIK和SMILE手术后提取的眼组织中的早期蛋白质谱。在对侧眼中进行SMILE和LASIK手术，并从5位捐献者的10只眼中收集基质组织样品。在LC-MS / MS中使用无标记定量方法和ITRAQ标记方法分析了基质组织样品。组合功能分析揭示了许多差异表达的蛋白质，这些蛋白质参与了重要的生物学过程。使用两种不同的蛋白质组学方法，鉴定出约117种独特的差异表达蛋白质。与非手术对照组相比，胶原蛋白，蛋白聚糖，角膜结晶蛋白富集并在SMILE和LASIK中显示差异表达。除此之外，还有14-3-3类蛋白质，溶菌酶（LYZ），巨噬细胞迁移抑制因子蛋白质（MIF），当在LASIK和SMILE之间进行比较时，色素上皮衍生因子（PEDF）差异表达。发现与SMILE相比，LASIK中的Peroxiredoxin 1（PRDX1）表达降低。与LASIK相比，发现SMILE中溶菌酶C和巨噬细胞迁移抑制因子炎症反应的表达较少。对特定标记（如胶原蛋白IV（COL4），角蛋白聚糖（KERA），Lumican（LUM），醛脱氢酶3 A1（ALDH3A1），溶菌酶C（LYZC））的蛋白质印迹验证证实了SMILE和LASIK操作的蛋白质水平存在差异与非手术对照相比。总之，这项研究揭示了这两种手术方法在角膜中发生的早期分子变化，这可能对术后并发症的处理有影响。