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Multi-omics analysis revealed that MAPK signaling and flavonoid metabolic pathway contributed to resistance against Meloidogyne incognita in the introgression line cucumber.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-01-28 , DOI: 10.1016/j.jprot.2020.103675 Xing Wang 1 , Chunyan Cheng 1 , Qingrong Li 1 , Kaijing Zhang 1 , Qunfeng Lou 1 , Ji Li 1 , Jinfeng Chen 1
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-01-28 , DOI: 10.1016/j.jprot.2020.103675 Xing Wang 1 , Chunyan Cheng 1 , Qingrong Li 1 , Kaijing Zhang 1 , Qunfeng Lou 1 , Ji Li 1 , Jinfeng Chen 1
Affiliation
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Inhibiting giant cells (GCs) development is an important characteristic of introgression line cucumber IL10-1 against Meloidogyne incognita proved by our previous study, but the systematic regulatory pathways were unknown. To reveal the regulation pathways more comprehensively, in the current study, we performed a joint analysis of RNA-Seq and lable-free quantitative proteomics between the IL10-1 (resistant) and the CC3 (susceptible cucumber) after inoculation with M. incognita. The results indicated that flavonoid biosynthesis pathway was specifically enriched in IL10-1. And protein species of Csa5P590220 and Csa5P589940 associated with flavonoid biosynthesis were highly translated in IL10-1 compared with these in CC3 at 3 days post inoculation (dpi), which would resulted in the excess of flavonoids in IL10-1 roots. In addition, phosphoproteomic analysis found that phosphorylated protein species involved in MAPK signaling cascade were enhanced in IL10-1, while they were inhibited in CC3 at 3 dpi. Accordingly, we speculate that the enhanced MAPK cascade signaling plays an important role in signal transduction for IL10-1 regulating the flavonoid biosynthesis. Knowledge from the study provide important regulatory pathways and protein species of introgression line cucumber against M. incognita, which will help in efforts to improve the recognition of the resistance mechanism of plants against nematode. SIGNIFICANCE: The current approach of joint analysis in transcription level, protein level and protein phosphorylation level more comprehensively revealed the different expression patterns at the molecular level of resistant and susceptible cucumber after inoculation with M. incognita. Based on the different expression patterns, we explore the pathway of resistance regulation of resistant cucumber IL10-1. Moreover, our results are helpful for the discovery of key genes and then apply them to M. incognita-resistance breeding.
中文翻译：
多组学分析表明，MAPK信号传导和类黄酮代谢途径有助于渗入黄瓜黄瓜对根结线虫的抗性。
先前的研究证明，抑制巨细胞（GCs）的发育是渗入黄瓜IL10-1抗根结线虫的重要特征，但是其系统调控途径尚不清楚。为了更全面地揭示调控途径，在本研究中，我们进行了隐孢子虫接种后IL10-1（抗性）和CC3（易感黄瓜）之间的RNA-Seq和无标签定量蛋白质组学的联合分析。结果表明，类黄酮生物合成途径在IL10-1中特异富集。接种后3天（dpi），与CC3中的类黄酮生物合成相关的Csa5P590220和Csa5P589940的蛋白种类在IL10-1中高度翻译，这将导致IL10-1根中的类黄酮过量。此外，磷酸蛋白质组学分析发现，IL10-1中增强了参与MAPK信号级联的磷酸化蛋白种类，而在3 dpi时CC3中则抑制了它们。因此，我们推测增强的MAPK级联信号传导在调节类黄酮生物合成的IL10-1的信号转导中起重要作用。该研究的知识提供了渗入黄瓜黄瓜对线虫的重要调控途径和蛋白质种类，这将有助于提高人们对植物对线虫的抗性机制的认识。意义：目前联合分析的转录水平，蛋白质水平和蛋白质磷酸化水平的方法更全面地揭示了接种M后黄瓜在抗性和易感黄瓜分子水平上的不同表达方式。隐身。根据不同的表达方式，我们探讨了抗性黄瓜IL10-1的抗性调控途径。此外，我们的结果有助于关键基因的发现，然后将其应用于抗隐杆线虫的育种。
更新日期：2020-01-30
中文翻译：
多组学分析表明，MAPK信号传导和类黄酮代谢途径有助于渗入黄瓜黄瓜对根结线虫的抗性。
先前的研究证明，抑制巨细胞（GCs）的发育是渗入黄瓜IL10-1抗根结线虫的重要特征，但是其系统调控途径尚不清楚。为了更全面地揭示调控途径，在本研究中，我们进行了隐孢子虫接种后IL10-1（抗性）和CC3（易感黄瓜）之间的RNA-Seq和无标签定量蛋白质组学的联合分析。结果表明，类黄酮生物合成途径在IL10-1中特异富集。接种后3天（dpi），与CC3中的类黄酮生物合成相关的Csa5P590220和Csa5P589940的蛋白种类在IL10-1中高度翻译，这将导致IL10-1根中的类黄酮过量。此外，磷酸蛋白质组学分析发现，IL10-1中增强了参与MAPK信号级联的磷酸化蛋白种类，而在3 dpi时CC3中则抑制了它们。因此，我们推测增强的MAPK级联信号传导在调节类黄酮生物合成的IL10-1的信号转导中起重要作用。该研究的知识提供了渗入黄瓜黄瓜对线虫的重要调控途径和蛋白质种类，这将有助于提高人们对植物对线虫的抗性机制的认识。意义：目前联合分析的转录水平，蛋白质水平和蛋白质磷酸化水平的方法更全面地揭示了接种M后黄瓜在抗性和易感黄瓜分子水平上的不同表达方式。隐身。根据不同的表达方式，我们探讨了抗性黄瓜IL10-1的抗性调控途径。此外，我们的结果有助于关键基因的发现，然后将其应用于抗隐杆线虫的育种。
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