Identifying factors bound to specific genomic loci in an unbiased manner is the holy grail of chromatin research, because it would provide an ultimate description of locus function. In this Comment, we explain why purifying a single locus represents a major technical challenge and provide recommendations for achieving this goal. Michiel Vermeulen and Jérôme Déjardin recommend how best to characterize the proteins that bind any specific genomic locus.

中文翻译：

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特定位点的染色质分离。

以无偏见的方式鉴定与特定基因组位点结合的因子是染色质研究的圣杯，因为它可以提供基因座功能的最终描述。在本评论中，我们解释了为何纯化单个基因座代表了一项重大技术挑战，并提供了实现此目标的建议。Michiel Vermeulen和JérômeDéjardin建议如何最好地表征结合任何特定基因组基因座的蛋白质。