Staphylococcus aureus Specific FRET Probe-Based Antibacterial Susceptibility Testing (SF-AST) by Detection of Micrococcal Nuclease Activity.,ACS Infectious Diseases

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Staphylococcus aureus Specific FRET Probe-Based Antibacterial Susceptibility Testing (SF-AST) by Detection of Micrococcal Nuclease Activity.
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2020-01-29 , DOI: 10.1021/acsinfecdis.9b00260
So Yeon Yi _{1,

2} , Jinyoung Jeong ₃ , Kyoon Eon Kim ₂ , Kyoungsook Park ₁ , Yong Beom Shin _{1,

4}

Affiliation

In this study, we describe a simple and rapid antibacterial susceptibility testing (AST) method for Staphylococcus aureus called S. aureus specific fluorescence resonance energy transfer (FRET) probe-based AST (SF-AST), which is based on an S. aureus specific FRET probe (SF probe) that detects micrococcal nuclease (MNase) activity secreted from S. aureus. The SF-AST was tested with an S. aureus quality control (QC) strain against six relevant antibiotics, and the minimum inhibitory concentration (MIC) values obtained with the broth microdilution (BMD) method were compared, as a gold standard AST. Results were obtained with high accuracy in 4-6 h. The MIC for the methicillin resistance using 20 clinical S. aureus isolates of SF-AST showed 100% sensitivity, specificity, positive predictive value, and negative predictive value, as compared to BMD. Thus, the SF-AST method is a simple, rapid, and useful antibiotic resistance test for S. aureus, and it provides a basis for clinical treatment in a short time.

中文翻译：

通过检测微球菌核酸酶活性，金黄色葡萄球菌特异性FRET探针为基础的抗菌药敏试验（SF-AST）。

在这项研究中，我们描述了一种简单快速的金黄色葡萄球菌抗菌药敏测试（AST）方法，称为金黄色葡萄球菌基于特异性荧光共振能量转移（FRET）探针的AST（SF-AST），该方法基于金黄色葡萄球菌特异性FRET探针（SF探针），用于检测金黄色葡萄球菌分泌的微球菌核酸酶（MNase）活性。SF-AST用金黄色葡萄球菌质量控制（QC）菌株针对六种相关抗生素进行了测试，并且比较了用肉汤微稀释（BMD）方法获得的最低抑菌浓度（MIC）值，作为金标准AST。在4-6小时内以高精度获得结果。与BMD相比，使用20株临床金黄色葡萄球菌SF-AST分离出的甲氧西林耐药性的MIC显示100％的敏感性，特异性，阳性预测值和阴性预测值。

更新日期：2020-01-29

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