Reproducible, scalable, and cost effective fabrication and versatile characterization of tissue spheroids (TS) is highly demanded by 3D bioprinting and drug discovery. Consistent geometry, defined mechanical properties, optimal viability, appropriate extracellular matrix/cell organization are required for cell aggregates aimed for application in these fields. A straightforward procedure for fabrication and systematic multiparametric characterization of TS with defined properties and uniform predictable geometry employing non-adhesive technology is suggested. Applying immortalized and primary cells, the reproducibility of spheroid generation, the strong correlation of ultimate spheroid diameter, and growth pattern with cell type and initial seeding concentration are demonstrated. Spheroids viability and mechanical properties are governed by cell derivation. In this study, a new decision procedure to apply for any cell type one starts to work with to prepare and typify TS meeting high quality standards in biofabrication and drug discovery is suggested.

中文翻译：

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从不同类型的细胞制造的组织球体的多参数分析。

3D生物打印和药物开发迫切需要可复制，可扩展且具有成本效益的制造方法，以及组织球体（TS）的通用特性。旨在在这些领域中应用的细胞聚集体需要一致的几何形状，确定的机械性能，最佳生存能力，适当的细胞外基质/细胞组织。建议使用非胶粘技术对具有定义的特性和统一的可预测几何形状的TS进行制造和系统化多参数表征的简单程序。应用永生细胞和原代细胞，显示了球状体生成的可再现性，最终球状体直径和生长模式与细胞类型和初始接种浓度的强相关性。球体的生存能力和机械性能受细胞衍生的支配。在这项研究中，提出了一种适用于任何一种细胞类型的新决策程序，开始着手准备和表征符合生物加工和药物发现中高质量标准的TS。