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Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-02-25 , DOI: 10.1111/jipb.12914 Yuekun Ren 1, 2 , Xiaojie Tian 1 , Shuyu Li 3 , Enyang Mei 1, 2 , Mingliang He 1, 2 , Jiaqi Tang 1, 2 , Min Xu 1, 2 , Xiufeng Li 1 , Zhenyu Wang 1 , Chuanyou Li 3, 4 , Qingyun Bu 1, 4
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-02-25 , DOI: 10.1111/jipb.12914 Yuekun Ren 1, 2 , Xiaojie Tian 1 , Shuyu Li 3 , Enyang Mei 1, 2 , Mingliang He 1, 2 , Jiaqi Tang 1, 2 , Min Xu 1, 2 , Xiufeng Li 1 , Zhenyu Wang 1 , Chuanyou Li 3, 4 , Qingyun Bu 1, 4
Affiliation
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Brassinosteroids (BRs) are plant‐specific steroid hormones which regulate plant growth, development, and adaptation. Transcriptional regulation plays key roles in plant hormone signaling. A mediator can serve as a bridge between gene‐specific transcription factors and the RNA polymerase machinery, functioning as an essential component in regulating the transcriptional process. However, whether a mediator is involved in BR signaling is unknown. Here, we discovered that Oryza sativa mediator subunit 25 (OsMED25) is an important regulator of rice BR signaling. Phenotypic analyses showed that the OsMED25‐RNAi and osmed25 mutant presented erect leaves, as observed in BR‐deficient mutants. In addition, the OsMED25‐RNAi and osmed25 mutant exhibited decreased BR sensitivity. Genetic analysis indicated that OsMED25‐RNAi could suppress the enhanced BR signaling phenotype of Osbzr1‐D. Further biochemical analysis showed that OsMED25 interacts with OsBZR1 in vivo, and OsMED25 is enriched on the promoter of OsBZR1 target genes. RNA sequencing analysis indicated that OsMED25 affects the expression of approximately 45% of OsBZR1‐regulated genes and mainly functions as a corepressor of OsBZR1. Together, these findings revealed that OsMED25 regulates rice BR signaling by interacting with OsBZR1 and modulating the expression of OsBZR1 target genes, thus expanding our understanding of the roles of mediators in plant hormone signaling.
中文翻译：
水稻介体亚基OsMED25与OsBZR1相互作用，调节油菜素类固醇信号传导和水稻的植物结构。
油菜素类固醇（BRs）是植物特有的类固醇激素，可调节植物的生长，发育和适应。转录调控在植物激素信号传导中起关键作用。介体可以充当基因特异性转录因子与RNA聚合酶机制之间的桥梁，在调节转录过程中起重要作用。但是，未知中介人是否参与BR信令。在这里，我们发现水稻水稻介体亚基25（OsMED25）是水稻BR信号的重要调控因子。表型分析表明，如在BR缺陷型突变体中观察到的，OsMED25-RNAi和osmed25突变体呈现出直立的叶子。此外，OsMED25‐RNAi和osmed25突变体表现出降低的BR敏感性。遗传分析表明，OsMED25‐RNAi可以抑制Osbzr1-D增强的BR信号表型。进一步的生化分析表明，OsMED25在体内与OsBZR1相互作用，并且OsMED25富含OsBZR1目标基因的启动子。RNA测序分析表明，OsMED25影响约45％的OsBZR1调控基因的表达，并且主要起OsBZR1的核心加压因子的作用。在一起，这些发现揭示了OsMED25通过与OsBZR1相互作用并调节OsBZR1靶基因的表达来调节水稻BR信号传导，从而扩展了我们对植物激素信号传导介体作用的理解。
更新日期：2020-02-25
中文翻译：
水稻介体亚基OsMED25与OsBZR1相互作用，调节油菜素类固醇信号传导和水稻的植物结构。
油菜素类固醇（BRs）是植物特有的类固醇激素，可调节植物的生长，发育和适应。转录调控在植物激素信号传导中起关键作用。介体可以充当基因特异性转录因子与RNA聚合酶机制之间的桥梁，在调节转录过程中起重要作用。但是，未知中介人是否参与BR信令。在这里，我们发现水稻水稻介体亚基25（OsMED25）是水稻BR信号的重要调控因子。表型分析表明，如在BR缺陷型突变体中观察到的，OsMED25-RNAi和osmed25突变体呈现出直立的叶子。此外，OsMED25‐RNAi和osmed25突变体表现出降低的BR敏感性。遗传分析表明，OsMED25‐RNAi可以抑制Osbzr1-D增强的BR信号表型。进一步的生化分析表明，OsMED25在体内与OsBZR1相互作用，并且OsMED25富含OsBZR1目标基因的启动子。RNA测序分析表明，OsMED25影响约45％的OsBZR1调控基因的表达，并且主要起OsBZR1的核心加压因子的作用。在一起，这些发现揭示了OsMED25通过与OsBZR1相互作用并调节OsBZR1靶基因的表达来调节水稻BR信号传导，从而扩展了我们对植物激素信号传导介体作用的理解。
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