Novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis.,Food Science & Nutrition

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Novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides released from quinoa protein by in silico proteolysis.
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-01-27 , DOI: 10.1002/fsn3.1423
Huimin Guo _{1,

2} , Aurore Richel ₂ , Yuqiong Hao ₁ , Xin Fan _{1,

2} , Nadia Everaert ₂ , Xiushi Yang ₁ , Guixing Ren ₁

Affiliation

Quinoa protein has been paid more and more attention because of its nutritional properties and beneficial effects. With the development of bioinformatics, bioactive peptide database and computer‐assisted simulation provide an efficient and time‐saving method for the theoretical estimation of potential bioactivities of protein. Therefore, the potential of quinoa protein sequences for releasing bioactive peptides was evaluated using the BIOPEP database, which revealed that quinoa protein, especially globulin, is a potential source of peptides with dipeptidyl peptidase‐IV (DPP‐IV) and angiotensin‐I‐converting enzyme (ACE) inhibitory activities. Three plant proteases, namely papain, ficin, and stem bromelain, were employed for the in silico proteolysis of quinoa protein. Furthermore, four tripeptides (MAF, NMF, HPF, and MCG) were screened as novel promising bioactive peptides by PeptideRanker. The bioactivities of selected peptides were confirmed using chemical synthesis and in vitro assay. The present work suggests that quinoa protein can serve as a good source of bioactive peptides, and in silico approach can provide theoretical assistance for investigation and production of functional peptides.

中文翻译：

通过计算机蛋白水解从藜麦蛋白中释放新型二肽基肽酶-IV 和血管紧张素-I 转换酶抑制肽。

藜麦蛋白因其营养特性和有益作用而受到越来越多的关注。随着生物信息学的发展，生物活性肽数据库和计算机辅助模拟为蛋白质潜在生物活性的理论估计提供了一种高效、省时的方法。因此，使用BIOPEP数据库评估了藜麦蛋白序列释放生物活性肽的潜力，结果表明藜麦蛋白，尤其是球蛋白，是具有二肽基肽酶-IV（DPP-IV）和血管紧张素-I转换肽的潜在来源酶（ACE）抑制活性。三种植物蛋白酶，即木瓜蛋白酶、无花果蛋白酶和茎菠萝蛋白酶，被用于藜麦蛋白的计算机蛋白水解。此外，PeptideRanker 筛选出四种三肽（MAF、NMF、HPF 和 MCG）作为新型有前景的生物活性肽。使用化学合成和体外测定证实了所选肽的生物活性。目前的工作表明藜麦蛋白可以作为生物活性肽的良好来源，并且计算机方法可以为功能肽的研究和生产提供理论帮助。

更新日期：2020-01-27

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