Herein, we describe the covalent attachment of resveratrol, a naturally occurring antioxidant, to the surface of stainless‐steel as a model for designing a novel bare‐metal stent to treat coronary artery disease. Resveratrol has been shown to reduce oxidative stress in dysfunctional endothelial cells, and stimulate arterial healing. Resveratrol treatments, however, are limited by low water solubility, such that a localized delivery to the site of arterial narrowing via a coated stent presents a promising strategy for improving stent outcomes. Our attachment strategy utilizes zirconium vapor deposition to lay down a thin layer of zirconium oxide with labile hydrocarbon groups at the surface. Resveratrol can displace these hydrocarbons in aprotic solvent to afford a covalently attached layer of resveratrol. We evaluated the release of resveratrol under a range of pH levels, including physiological conditions (pH = 7.4 and 37 °C). Furthermore, we established that endothelial cells grown on a resveratrol‐bound surface release elevated nitric oxide levels compared to controls, a key endothelial signaling molecule responsible for arterial health. These results are promising toward the development of a resveratrol‐coated bare‐metal stent to improve patient outcomes.

中文翻译：

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白藜芦醇与不锈钢的共价连接以开发释放白藜芦醇的裸金属支架。

在此，我们描述了白藜芦醇（一种天然抗氧化剂）与不锈钢表面的共价连接，作为设计治疗冠状动脉疾病的新型裸金属支架的模型。白藜芦醇已被证明可以减少功能失调的内皮细胞的氧化应激，并刺激动脉愈合。然而，白藜芦醇治疗受到低水溶性的限制，因此通过涂层支架局部输送到动脉狭窄部位是改善支架效果的有前途的策略。我们的附着策略利用锆气相沉积在表面沉积一层带有不稳定烃基的氧化锆薄层。白藜芦醇可以在非质子溶剂中置换这些碳氢化合物，以提供共价连接的白藜芦醇层。我们评估了白藜芦醇在一系列 pH 水平下的释放，包括生理条件（pH = 7.4 和 37 °C）。此外，我们确定与对照相比，在白藜芦醇结合表面上生长的内皮细胞释放出升高的一氧化氮水平，而对照是负责动脉健康的关键内皮信号分子。这些结果有望用于开发白藜芦醇涂层裸金属支架以改善患者预后。