The development of polymeric nanocomposites for biomedical applications remains a major challenge in terms of tailored addition of nanoparticles to realize the simultaneous enhancement of fracture resistance and cell/blood compatibility. To address this, the present work has been planned to determine whether small addition of surface functionalized multiwalled‐carbon‐nanotube, MWCNT (<1.5 wt%) and egg‐shell derived nanosized hydroxyapatite, nHA (<10 wt%) to ultrahigh‐molecular‐weight‐polyethylene (UHMWPE) can significantly improve the physical properties as well as biocompatibility. The difference in mouse osteoblast and human mesenchymal stem cell (hMSc) proliferation has been validated using both the monolithic composite and a trilayered composite with two different UHMWPE nanocomposites on either face with pure polymer at the middle. The combination of rheology and micro‐CT with fractography reveals the homogeneous dispersion of nanofillers, leading to mechanical property enhancement. The quantitative analysis of cell viability and cell spreading by immunocytochemistry method, using vinculin and vimentin expression, establish significant cytocompatibility with hMSc and osteoblast cells onto the trilayer hybrid nanobiocomposite substrates. The hemocompatibility of the investigated composites under the controlled flow of rabbit blood in a microfluidic device reveals the signature of reduced thrombogenesis with reduction of platelet activation on UHMWPE nanocomposite w.r.t. unreinforced UHMWPE. An attempt has been made to discuss the blood compatibility results in the backdrop of the bovine serum albumin adsorption kinetics. Summarizing, the present study establishes that the twin requirement of mechanical property and cyto/hemo‐compatibility can be potentially realized in developing trilayer composites in UHMWPE‐nHA‐MWCNT system.

中文翻译：

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基于 UHMWPE-MWCNT-nHA 的混合三层纳米生物复合材料：加工方法、物理特性、干/骨细胞功能和血液相容性。

开发用于生物医学应用的聚合物纳米复合材料在定制添加纳米颗粒以实现抗断裂性和细胞/血液相容性的同时增强方面仍然是一个主要挑战。为了解决这个问题，目前的工作已计划确定是否将表面功能化的多壁碳纳米管、MWCNT（<1.5 wt%）和蛋壳衍生的纳米羟基磷灰石、nHA（<10 wt%）添加到超高分子量碳纳米管中。重量聚乙烯（UHMWPE）可以显着改善物理性能和生物相容性。小鼠成骨细胞和人类间充质干细胞 (hMSc) 增殖的差异已使用整体复合材料和三层复合材料进行了验证，该复合材料的任一面上都有两种不同的 UHMWPE 纳米复合材料，中间为纯聚合物。流变学和显微 CT 与断口相结合，揭示了纳米填料的均匀分散，从而提高了机械性能。通过免疫细胞化学方法对细胞活力和细胞扩散进行定量分析，使用纽蛋白和波形蛋白表达，建立了与 hMSc 和成骨细胞在三层混合纳米复合基质上的显着细胞相容性。在微流体装置中受控兔血流量下所研究的复合材料的血液相容性揭示了在 UHMWPE 纳米复合材料与未增强的 UHMWPE 上，血栓形成减少和血小板活化减少的特征。已经尝试在牛血清白蛋白吸附动力学的背景下讨论血液相容性结果。总结一下，