Newly developed potato varieties in Japan have never replaced existing old varieties, indicating genetic poverty in our gene pool. To broaden the genetic diversity, Andean primitive cultivated potatoes (Andigena) were used to create parental clones. A total of 184 Andigena accessions and 10 tetraploid Solanum tarijense-Andigena hybrid clones were crossed with a breeding clone 10H17. From 6939 F₁ hybrid clones, 287 were selected as long-day-adapted F₁ clones. From progeny evaluation using these as pollen parents crossed with Atlantic, 95 male-fertile and high-yielding F₁ clones were selected (PGEL clones). The genetic relationship was evaluated using 17,947 single nucleotide polymorphism (SNP) markers, which disclosed their distinctiveness, with larger mean Euclidean distances between PGEL clones and Japanese, foreign, or all tetraploid varieties (48.0, 49.9 or 49.4, respectively) than between Japanese and foreign varieties (47.2). Thus, these PGEL clones can be used to expand genetic diversity in Japanese potato breeding populations.

中文翻译：

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扩大日本马铃薯基因库的遗传多样性

日本的新开发的马铃薯品种从未取代现有的旧品种，这表明我们的基因库中存在遗传贫困。为了扩大遗传多样性，使用了安第斯原始栽培马铃薯（Andigena）创建了亲本克隆。将总共​​184个Andigena登录号和10个四倍体塔兰茄-Andigena杂交克隆与育种克隆10H17杂交。从6939个F ₁杂种克隆中，选择287个作为经过长时间适应的F ₁克隆。通过使用这些作为花粉亲本的后代评估，他们与大西洋杂交，有95个雄性可育且高产的F ₁选择克隆（PGEL克隆）。使用17,947个单核苷酸多态性（SNP）标记评估了遗传关系，该标记揭示了它们的独特性，PGEL克隆与日本，国外或所有四倍体变种（分别为48.0、49.9或49.4）之间的平均欧几里德距离比日本和日本之间的大。外国品种（47.2）。因此，这些PGEL克隆可用于扩大日本马铃薯育种种群的遗传多样性。