Eggplant is an important Solanaceous vegetable crop with distinct morphological forms, colours, and shape of the fruits. Limited genomic coverage of the crop is a major constraint to unfold the cause of morphological variations underlying the contrasting traits. Therefore, with the aim to initiate targeted breeding efforts and to render ready-to-use markers for application in mapping quantitative trait loci (QTLs)/genes and to facilitate MAS, arbitrary and microsatellite molecular markers were used to map an inter-specific mapping population of 114 F₈-recombinant inbred lines (RILs) developed using two contrasting lines, Ramnagar Giant (Solanum melongena L.) and W-4 (S. incanum L.). The arbitrary and microsatellite markers were screened for polymorphism with aim to cover the whole genome of eggplant. Of the total 1443 number of markers investigated, 6.07% RAPDs, 25% ISSR, 46.25% SCoT, and 22.46% SSRs revealed polymorphism between the parental lines, and were used for genotyping the RILs population. The results exhibit robust and sturdy nature of SCoT and microsatellite markers in unwrapping variations between both genotypes. Further, a high-density genetic linkage map was constructed with LOD threshold ≥ 4 clustered into 12 tentative linkage groups and spanning 1165 cM of the eggplant genome with an average marker density of 4.13 cM. The results shall further be used in mapping QTLs of agri-horticultural traits in eggplant.

中文翻译：

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基于任意和微卫星标记的茄子种间重组自交系的高密度遗传连锁图谱（Solanum melongena L.）

茄子是一种重要的茄类蔬菜作物，具有独特的形态，颜色和果实形状。有限的农作物基因组覆盖是揭示潜在特征之间形态变异的主要原因。因此，为了启动有针对性的育种工作并提供可用于在定量性状基因座（QTL）/基因作图时使用的标记，并为了促进MAS，使用任意和微卫星分子标记作图作种间作图使用两个对比系Ramnagar Giant（Solanum melongena L.）和W-4（S. incanum）发育的114 F ₈重组自交系（RIL）群体L.）。筛选任意和微卫星标记的多态性，以覆盖茄子的整个基因组。在总共研究的1443个标记中，有6.07％的RAPD，25％的ISSR，46.25％的SCoT和22.46％的SSR揭示了亲本品系之间的多态性，并用于对RILs群体进行基因分型。结果在两种基因型之间的解链变异中表现出SCoT和微卫星标记的稳健性。此外，构建了LOD阈值≥4的高密度遗传连锁图谱，将其分为12个暂定连锁组，跨越了茄子基因组的1165 cM，平均标记密度为4.13 cM。该结果应进一步用于定位茄子农业园艺性状的QTL。