Laccase is a versatile multicopper oxidase that holds great promise for many biotechnological applications. For such applications, it is essential to explore good biocatalytic systems for high activity and recyclability. The feasibility of membrane enclosed enzymatic catalysis (MEEC) for enzyme recycling with laccase was evaluated. The dialysis membrane enclosed laccase catalysis (DMELC) was tested for the conversion of the non-phenolic model substrate 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Trametes versicolor laccase was found to be completely retained by the dialysis membrane during the process. The ABTS total conversion after DMELC reached the same values as the batch reaction of the enzyme in solution. The efficiency of DMELC conversion of ABTS under different process conditions including shaking speed, temperature, ABTS concentration and pH was investigated. The repetitive dialysis minimally affected the activity and the protein content of the enclosed laccase. DMELC retained 70.3 ± 0.8% of its initial conversion after 5 cycles. The usefulness of MEEC extends to other enzymes with the benefit of superior activity of an enzyme in solution and the recyclability which is normally only obtained with immobilized enzymes.

中文翻译：

---

透析膜封闭的漆酶催化结合了可控制的转化率和可回收性，而无需固定酶。

漆酶是一种多功能的多铜氧化酶，在许多生物技术应用中都具有广阔的前景。对于此类应用，必须探索具有高活性和可回收性的良好生物催化系统。评估了膜封闭酶催化（MEEC）用于漆酶再生的可行性。测试了透析膜封闭的漆酶催化（DMELC）的非酚类模型底物2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）的转化。发现在此过程中，trametes versicolor漆酶完全被透析膜保留。DMELC之后的ABTS总转化率与溶液中酶的间歇反应达到相同的值。ABTS在不同工艺条件（包括振荡速度，温度，研究了ABTS的浓度和pH。重复透析对封闭的漆酶的活性和蛋白质含量的影响最小。5个循环后，DMELC保留了其初始转化率的70.3±0.8％。MEEC的用途扩展到其他酶，其优点是酶在溶液中具有出色的活性，并且可回收性通常仅通过固定化的酶才能获得。