Myrrh is the resinous exudate obtained by the incision in Commiphora molmol trees (Family Burseraceae). The bactericidal activity of its hexane extract was compared to its essential oil (MEO) using viable count technique against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (Ps. aeruginosa). MEO exhibited a better activity with > 99.999% killing of both tested strains after 2 h contact time. MEO was tested using the same technique against four multidrug resistant isolates: S. aureus (MRSA, sputum), Escherichia coli (E. coli, urine), Ps. aeruginosa (wound) and Klebsiella pneumonia (K. pneumonia, sputum). Highest bactericidal activity was observed against Ps. aeruginosa while lowest was against K. pneumonia (99.59 and 54.04% killing, respectively after 2 h contact time). A cream and mouthwash were formulated using 5% v/v MEO. The cream showed a better activity against Ps. aeruginosa than S. aureus (95.11 and 86.76% killing, respectively after 2 h contact time). The in vitro treatment of ca 107 CFU/ml S. aureus cells suspended in 10% saliva with the mouthwash produced ca 46% killing within the first 15 min reaching ca 99.999% after 30 min. Cytotoxic studies of both the essential oil and hexane extract on human liver cancer (Hep G2), human breast cancer (MCF-7) and colon cancer cell lines (HCT-116) revealed a promising in vitro activity. Highest activity was recorded for the essential oil on MCF-7 with IC50 10.93 ± 0.32 μg/ml. GC/MS analysis allowed the identification of 17 and 9 compounds representing 92.01 and 99.99% of the hexane extract and essential oil, respectively. Furano-eudesma-1,3-diene (15.99%) and 2-acetoxy-furano-diene (26.82%) were the major identified compounds in the hexane extract and essential oil, respectively. These results indicate that Myrrh essential oil is a promising antibacterial and cytotoxic agent that can be formulated in suitable dosage forms.

中文翻译：

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没药提取物和两种剂型对标准细菌菌株和具有GC / MS分析的耐多药临床分离株的杀菌活性。

没药是通过在孔雀科（Burseraceae）科中的切口获得的树脂状分泌物。使用可行的计数技术，将其己烷提取物的杀菌活性与其精油（MEO）进行比较，以对抗金黄色葡萄球菌（S. aureus）和铜绿假单胞菌（Ps。aeruginosa）。接触2小时后，MEO表现出更好的活性，两种测试菌株的杀伤率均> 99.999％。使用相同的技术对四种耐多药菌株进行了MEO测试：金黄色葡萄球菌（MRSA，痰），大肠杆菌（大肠杆菌，尿液），PS。铜绿假单胞菌（伤口）和肺炎克雷伯菌（K. pneumonia，痰）。观察到对Ps具有最高的杀菌活性。铜绿假单胞菌对肺炎克雷伯菌的影响最低（接触2小时后分别杀灭99.59和54.04％）。使用5％v / v MEO配制乳膏和漱口水。该乳霜显示出更好的抗Ps活性。铜绿假单胞菌比金黄色葡萄球菌（接触2 h后分别杀死95.11和86.76％）。用漱口水对悬浮在10％唾液中的约107 CFU / ml金黄色葡萄球菌细胞进行体外处理，在前15分钟内杀死率约为46％，在30分钟后达到约99.999％。精油和己烷提取物对人肝癌（Hep G2），人乳腺癌（MCF-7）和结肠癌细胞系（HCT-116）的细胞毒性研究显示，其体外活性很有希望。香精油在MCF-7上的最高活性记录为IC50 10.93±0.32μg/ ml。GC / MS分析可以鉴定出17和9种化合物，分别占己烷提取物和精油的92.01％和99.99％。呋喃诺-阿德斯马-1,3-二烯（15.99％）和2-乙酰氧基-呋喃二烯（26。82％）分别是己烷提取物和精油中主要鉴定出的化合物。这些结果表明没药精油是一种有前途的抗菌和细胞毒剂，可以配制成合适的剂型。