The purpose of the study is to investigate the molecular mechanisms of development of Tricholoma matsutake fruiting body at the primordial stage (TM-1), the intermediate stage (TM-2) and the mature stage (TM-3) using RNA-Seq sequencing technology. The analysis of gene expression level revealed that the Spn2 and Eef1a1 gene were the key genes in the primordial stage of T. matsutake by regulating cytokinesis, protein synthesis, and cell growth. And the Ubc, Atp6, Cytb, and Pth2 gene were the key genes in the mature stage of T. matsutake by regulating energy metabolism and protein synthesis. Differential expression genes (DEGs) analysis results showed that Cdc28, Rad53, Dun1, Pho85 and Pho81 were the key DEGs regulating cell cycle genes of T. matsutake from primordial stage to intermediate stage. And APC, Cyr1, Cdc45, Spo11 and Rec8 genes were the key DEGs for the meiosis and sporogenesis of T. matsutake from the intermediate stage to the mature stage.

本研究的目的是使用RNA-Seq测序技术研究松茸口蘑子实体在原始阶段（TM-1），中间阶段（TM-2）和成熟阶段（TM-3）发育的分子机制。技术。基因表达水平的分析表明，通过调节胞质分裂，蛋白质合成和细胞生长，Spn2和Eef1a1基因是松茸原始阶段的关键基因。而UBC，ATP6，细胞色素b，和PTH2基因是在成熟阶段的关键基因T.松茸通过调节能量代谢和蛋白质合成。差异表达基因（DEGs）分析结果表明，Cdc28，Rad53，Dun1，Pho85和Pho81是调控松茸从原始阶段到中期的细胞周期基因的关键DEG 。而APC，Cyr1，Cdc45，Spo11和Rec8基因是从中间阶段到成熟阶段松茸减数分裂和孢子发生的关键DEG 。