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Anaerobic methane oxidation coupled to manganese reduction by members of the Methanoperedenaceae.
The ISME Journal ( IF 10.8 ) Pub Date : 2020-01-27 , DOI: 10.1038/s41396-020-0590-x Andy O Leu 1 , Chen Cai 2 , Simon J McIlroy 1 , Gordon Southam 3 , Victoria J Orphan 4 , Zhiguo Yuan 2 , Shihu Hu 2 , Gene W Tyson 1
The ISME Journal ( IF 10.8 ) Pub Date : 2020-01-27 , DOI: 10.1038/s41396-020-0590-x Andy O Leu 1 , Chen Cai 2 , Simon J McIlroy 1 , Gordon Southam 3 , Victoria J Orphan 4 , Zhiguo Yuan 2 , Shihu Hu 2 , Gene W Tyson 1
Affiliation
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Anaerobic oxidation of methane (AOM) is a major biological process that reduces global methane emission to the atmosphere. Anaerobic methanotrophic archaea (ANME) mediate this process through the coupling of methane oxidation to different electron acceptors, or in concert with a syntrophic bacterial partner. Recently, ANME belonging to the archaeal family Methanoperedenaceae (formerly known as ANME-2d) were shown to be capable of AOM coupled to nitrate and iron reduction. Here, a freshwater sediment bioreactor fed with methane and Mn(IV) oxides (birnessite) resulted in a microbial community dominated by two novel members of the Methanoperedenaceae, with biochemical profiling of the system demonstrating Mn(IV)-dependent AOM. Genomic and transcriptomic analyses revealed the expression of key genes involved in methane oxidation and several shared multiheme c-type cytochromes (MHCs) that were differentially expressed, indicating the likely use of different extracellular electron transfer pathways. We propose the names "Candidatus Methanoperedens manganicus" and "Candidatus Methanoperedens manganireducens" for the two newly described Methanoperedenaceae species. This study demonstrates the ability of members of the Methanoperedenaceae to couple AOM to the reduction of Mn(IV) oxides, which suggests their potential role in linking methane and manganese cycling in the environment.
中文翻译：
厌氧甲烷氧化与甲烷操作科成员的锰还原相结合。
甲烷厌氧氧化（AOM）是减少全球甲烷排放到大气中的主要生物过程。厌氧甲烷氧化古菌（ANME）通过将甲烷氧化与不同的电子受体耦合，或与互养细菌伙伴协同介导这一过程。最近，属于古菌科 Methanoperedenaceae 的 ANME（以前称为 ANME-2d）被证明能够将 AOM 与硝酸盐和铁还原偶联。在这里，用甲烷和锰（IV）氧化物（水钠锰矿）供给的淡水沉积物生物反应器形成了一个以甲烷操作科的两个新成员为主的微生物群落，系统的生化分析表明了锰（IV）依赖性AOM。基因组和转录组分析揭示了参与甲烷氧化的关键基因的表达以及差异表达的几种共享的多血红素c型细胞色素（MHC），表明可能使用不同的细胞外电子转移途径。我们建议将两个新描述的 Methanoperedenaceae 物种命名为“Candidatus Methanoperedens manganicus”和“Candidatus Methanoperedens manganireducens”。这项研究证明了甲烷操作科成员将 AOM 与 Mn(IV) 氧化物还原耦合的能力，这表明它们在连接环境中甲烷和锰循环方面的潜在作用。
更新日期：2020-01-29
中文翻译：
厌氧甲烷氧化与甲烷操作科成员的锰还原相结合。
甲烷厌氧氧化（AOM）是减少全球甲烷排放到大气中的主要生物过程。厌氧甲烷氧化古菌（ANME）通过将甲烷氧化与不同的电子受体耦合，或与互养细菌伙伴协同介导这一过程。最近，属于古菌科 Methanoperedenaceae 的 ANME（以前称为 ANME-2d）被证明能够将 AOM 与硝酸盐和铁还原偶联。在这里，用甲烷和锰（IV）氧化物（水钠锰矿）供给的淡水沉积物生物反应器形成了一个以甲烷操作科的两个新成员为主的微生物群落，系统的生化分析表明了锰（IV）依赖性AOM。基因组和转录组分析揭示了参与甲烷氧化的关键基因的表达以及差异表达的几种共享的多血红素c型细胞色素（MHC），表明可能使用不同的细胞外电子转移途径。我们建议将两个新描述的 Methanoperedenaceae 物种命名为“Candidatus Methanoperedens manganicus”和“Candidatus Methanoperedens manganireducens”。这项研究证明了甲烷操作科成员将 AOM 与 Mn(IV) 氧化物还原耦合的能力，这表明它们在连接环境中甲烷和锰循环方面的潜在作用。
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